BACKGROUND: Changes in concentration of thyroid hormones can affect Na+-K+-ATPase number and activity and phospholipid composition of the cell membranes leading to changes in the surface to volume ratio and strength of membrane. AIM: In this study, the osmotic fragility of the red blood cells from non-treated hypo- and hyperthyroid patients was compared to that of control subjects. MATERIAL/SUBJECTS AND METHODS: After 3 washings with normal saline, red blood cells were placed in varying concentrations of sodium chloride (Na- Cl) (0-0.9%) and fragility was assessed with colorimetric method; to do this, after the incubation period, tubes were centrifuged and the optical density of the tubes was measured. Hemolysis percentage in tubes was calculated based on 100% hemolysis in the tubes containing no NaCl (0%). RESULTS: Osmotic fragility of the cells from hyperthyroid patients in 0.45% NaCl was significantly lower than control subjects (74.6%+/-30.2 vs 93.8%+/-9.1, p<0.01). The osmotic fragility of red blood cells in 0.5% concentration of sodium chloride in hyperthyroid patients was significantly lower compared to that of controls (27.8%+/-26.0 vs 63.5%+/-27.5, p<0.001). No significant difference was observed between the osmotic fragility of the hypothyroid patients compared with control subjects. CONCLUSIONS: Alteration in osmotic fragility is seen in patients with hyperthyroidism; however, anemia reported in hypo- or hyperthyroid patients is not due to high osmotic fragility of red blood cells and other causes need to be investigated.
BACKGROUND: Changes in concentration of thyroid hormones can affect Na+-K+-ATPase number and activity and phospholipid composition of the cell membranes leading to changes in the surface to volume ratio and strength of membrane. AIM: In this study, the osmotic fragility of the red blood cells from non-treated hypo- and hyperthyroidpatients was compared to that of control subjects. MATERIAL/SUBJECTS AND METHODS: After 3 washings with normal saline, red blood cells were placed in varying concentrations of sodium chloride (Na- Cl) (0-0.9%) and fragility was assessed with colorimetric method; to do this, after the incubation period, tubes were centrifuged and the optical density of the tubes was measured. Hemolysis percentage in tubes was calculated based on 100% hemolysis in the tubes containing no NaCl (0%). RESULTS: Osmotic fragility of the cells from hyperthyroidpatients in 0.45% NaCl was significantly lower than control subjects (74.6%+/-30.2 vs 93.8%+/-9.1, p<0.01). The osmotic fragility of red blood cells in 0.5% concentration of sodium chloride in hyperthyroidpatients was significantly lower compared to that of controls (27.8%+/-26.0 vs 63.5%+/-27.5, p<0.001). No significant difference was observed between the osmotic fragility of the hypothyroidpatients compared with control subjects. CONCLUSIONS: Alteration in osmotic fragility is seen in patients with hyperthyroidism; however, anemia reported in hypo- or hyperthyroidpatients is not due to high osmotic fragility of red blood cells and other causes need to be investigated.
Authors: E Justo Firvida; S Maceda Vilariño; F Lado Lado; J R Devesa Barreira; A San Miguel Hernández; E G Torreiro Journal: An Med Interna Date: 1995-09
Authors: Daisy M Wopereis; Robert S Du Puy; Diana van Heemst; John P Walsh; Alexandra Bremner; Stephan J L Bakker; Douglas C Bauer; Anne R Cappola; Graziano Ceresini; Jean Degryse; Robin P F Dullaart; Martin Feller; Luigi Ferrucci; Carmen Floriani; Oscar H Franco; Massimo Iacoviello; Georgio Iervasi; Misa Imaizumi; J Wouter Jukema; Kay-Tee Khaw; Robert N Luben; Sabrina Molinaro; Matthias Nauck; Kushang V Patel; Robin P Peeters; Bruce M Psaty; Salman Razvi; Roger K Schindhelm; Natasja M van Schoor; David J Stott; Bert Vaes; Mark P J Vanderpump; Henry Völzke; Rudi G J Westendorp; Nicolas Rodondi; Christa M Cobbaert; Jacobijn Gussekloo; Wendy P J den Elzen Journal: J Clin Endocrinol Metab Date: 2018-10-01 Impact factor: 5.958