BACKGROUND: Diabetic nephropathy is a leading cause of end-stage renal disease. Premature mortality is common in patients with nephropathy, largely due to cardiovascular disease. Genetic variants implicated in macrovascular disease are therefore excellent candidates to assess for association with diabetic nephropathy. Recent genome-wide association studies have identified a total of 15 single-nucleotide polymorphisms (SNPs) that are reproducibly associated with cardiovascular disease. METHODS: We initially assessed these SNPs for association in UK type 1 diabetic patients with (cases; n = 597) and without (controls; n = 502) nephropathy using iPLEX(TM) and TaqMan(R) assays. Replication studies were performed with DNA genotyped in a total of 2668 individuals from the British Isles. RESULTS: One SNP (rs4420638) on chromosome 19q13 was found to be significantly associated with diabetic nephropathy before (P = 0.0002) and after correction for multiple testing (P(corrected) = 0.002). We replicated this finding in a phenotypically similar case-control collection comprising 709 individuals with type 1 diabetes (P = 0.002; combined P < 0.00001; OR = 1.54, 95% CI: 1.29-1.84). CONCLUSIONS: Our case-control data suggest that rs4420638, or a functional SNP in linkage disequilibrium with this SNP, may be associated with diabetic nephropathy.
BACKGROUND:Diabetic nephropathy is a leading cause of end-stage renal disease. Premature mortality is common in patients with nephropathy, largely due to cardiovascular disease. Genetic variants implicated in macrovascular disease are therefore excellent candidates to assess for association with diabetic nephropathy. Recent genome-wide association studies have identified a total of 15 single-nucleotide polymorphisms (SNPs) that are reproducibly associated with cardiovascular disease. METHODS: We initially assessed these SNPs for association in UK type 1 diabeticpatients with (cases; n = 597) and without (controls; n = 502) nephropathy using iPLEX(TM) and TaqMan(R) assays. Replication studies were performed with DNA genotyped in a total of 2668 individuals from the British Isles. RESULTS: One SNP (rs4420638) on chromosome 19q13 was found to be significantly associated with diabetic nephropathy before (P = 0.0002) and after correction for multiple testing (P(corrected) = 0.002). We replicated this finding in a phenotypically similar case-control collection comprising 709 individuals with type 1 diabetes (P = 0.002; combined P < 0.00001; OR = 1.54, 95% CI: 1.29-1.84). CONCLUSIONS: Our case-control data suggest that rs4420638, or a functional SNP in linkage disequilibrium with this SNP, may be associated with diabetic nephropathy.
Authors: E Fagerholm; E Ahlqvist; C Forsblom; N Sandholm; A Syreeni; M Parkkonen; A J McKnight; L Tarnow; A P Maxwell; H-H Parving; L Groop; P-H Groop Journal: Diabetologia Date: 2012-05-29 Impact factor: 10.122
Authors: Amy Jayne McKnight; Christopher C Patterson; Kerry A Pettigrew; David A Savage; Jill Kilner; Madeline Murphy; Denise Sadlier; Alexander P Maxwell Journal: J Am Soc Nephrol Date: 2010-02-11 Impact factor: 10.121
Authors: Gareth J McKay; David A Savage; Christopher C Patterson; Gareth Lewis; Amy Jayne McKnight; Alexander P Maxwell Journal: PLoS One Date: 2013-03-26 Impact factor: 3.240