Fang Wu1, Ying-Qi Liang, Zhi-Ming Huang. 1. Department of Gastroenterology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
Abstract
OBJECTIVE: To study the expression of DJ-1 in human hepatocellular carcinoma and its relationship with tumor invasion and metastasis. METHODS: Reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical methods were used to detect the expression of DJ-1 mRNA and protein in tumor tissue and para-tumor tissue of 46 cases of hepatocellular carcinoma. The relationship between the expression of DJ-1 and clinicopathologic parameters was analyzed. RESULTS: DJ-1 mRNA and protein were expressed in 69.6% and 58.7% of the tumor tissues, which were significantly higher than those in para-tumor tissues (39.1% and 34.8%), chi2 = 8.587, P < 0.05. The increased expression of DJ-1 protein was not correlated with sex of patients, size of tumor, AFP, HBsAg, differentiation level of tumor and hepatocirrhosis (P > 0.05), but with the capsula of tumor, thrombus in the portal vein (P < 0.05). CONCLUSIONS: DJ-1 gene expression may play an important role in the invasion and metastasis of HCC.
OBJECTIVE: To study the expression of DJ-1 in humanhepatocellular carcinoma and its relationship with tumor invasion and metastasis. METHODS: Reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical methods were used to detect the expression of DJ-1 mRNA and protein in tumor tissue and para-tumor tissue of 46 cases of hepatocellular carcinoma. The relationship between the expression of DJ-1 and clinicopathologic parameters was analyzed. RESULTS:DJ-1 mRNA and protein were expressed in 69.6% and 58.7% of the tumor tissues, which were significantly higher than those in para-tumor tissues (39.1% and 34.8%), chi2 = 8.587, P < 0.05. The increased expression of DJ-1 protein was not correlated with sex of patients, size of tumor, AFP, HBsAg, differentiation level of tumor and hepatocirrhosis (P > 0.05), but with the capsula of tumor, thrombus in the portal vein (P < 0.05). CONCLUSIONS:DJ-1 gene expression may play an important role in the invasion and metastasis of HCC.
Authors: Ali Nowrouzi; Wing T Cheung; Tingting Li; Xuegong Zhang; Anne Arens; Anna Paruzynski; Simon N Waddington; Emma Osejindu; Safia Reja; Christof von Kalle; Yoahe Wang; Faisal Al-Allaf; Lisa Gregory; Matthew Themis; Maxine Holder; Niraja Dighe; Alaine Ruthe; Suzanne Mk Buckley; Brian Bigger; Eugenio Montini; Adrian J Thrasher; Robert Andrews; Terry P Roberts; Robert F Newbold; Charles Coutelle; Manfred Schmidt; Mike Themis Journal: Mol Ther Date: 2012-11-13 Impact factor: 11.454