BACKGROUND: PRF treatment has recently been described as minimally neurodestructive alternative to radiofrequency heat lesions. Patients with some pain syndromes in whom the pain could not be controlled by alternative techniques may be treated using PRF. In the present study, our main goal was to evaluate and compare the ultrastructure of peripheral nerve tissue that was heated by PRF, CRF with 42 degrees C, and CRF with 70 degrees C. METHODS: Forty-five male rats were divided into 5 groups. In PRF group and CRF with 42 degrees C group, the sciatic nerve was heated at a temperature of 42 degrees C for 120 seconds. As a positive control, some rat sciatic nerves were treated with CRF lesions at 70 degrees C. The rats were kept alive for 21 days and then killed. Tissue was evaluated with transmission electron microscope, and grading was done to the groups. RESULTS: The unmyelinated nerve fibers were ultrastructurally normal in all groups. The results of myelinated axons indicated that PRF group had better grades, and CRF with 70 degrees C group had the worst grade. Especially, comparison of the group of PRF and CRF with 42 degrees C revealed significant difference. In PRF group, none of the myelinated axons showed severe degeneration findings, and most of the damaged myelinated axons showed only separation in myelin configuration. CONCLUSIONS: PRF treatment may cause separation in myelinated axons. However, it seems that all changes were reversible. The present study supports the hypothesis that pulsed RF treatment does not rely on thermal injury of neurologic tissue to achieve its effect.
BACKGROUND: PRF treatment has recently been described as minimally neurodestructive alternative to radiofrequency heat lesions. Patients with some pain syndromes in whom the pain could not be controlled by alternative techniques may be treated using PRF. In the present study, our main goal was to evaluate and compare the ultrastructure of peripheral nerve tissue that was heated by PRF, CRF with 42 degrees C, and CRF with 70 degrees C. METHODS: Forty-five male rats were divided into 5 groups. In PRF group and CRF with 42 degrees C group, the sciatic nerve was heated at a temperature of 42 degrees C for 120 seconds. As a positive control, some rat sciatic nerves were treated with CRF lesions at 70 degrees C. The rats were kept alive for 21 days and then killed. Tissue was evaluated with transmission electron microscope, and grading was done to the groups. RESULTS: The unmyelinated nerve fibers were ultrastructurally normal in all groups. The results of myelinated axons indicated that PRF group had better grades, and CRF with 70 degrees C group had the worst grade. Especially, comparison of the group of PRF and CRF with 42 degrees C revealed significant difference. In PRF group, none of the myelinated axons showed severe degeneration findings, and most of the damaged myelinated axons showed only separation in myelin configuration. CONCLUSIONS: PRF treatment may cause separation in myelinated axons. However, it seems that all changes were reversible. The present study supports the hypothesis that pulsed RF treatment does not rely on thermal injury of neurologic tissue to achieve its effect.
Authors: Danielle M Perret; Doo-Sik Kim; Kang-Wu Li; Karin Sinavsky; Robert L Newcomb; Jason M Miller; Z David Luo Journal: Anesth Analg Date: 2011-05-19 Impact factor: 5.108