Repair of segmental bone-defect of goat's tibia using a dynamic perfusion culture tissue engineering bone.

Segmental bone defect resulted from trauma or excision of bone tumor or pathology represents a common and significant clinical problem. In an attempt to solve this dilemma, we use beta-TCP combined with autologous bone marrow mesenchymal stem cells (auto-BMSC) and cultured by dynamic perfusion to repair the segmental bone defects of goat's tibia. The beta-TCP scaffolds combined with auto-BMSC by dynamic perfusion bioreactor or in static state were respectively transplanted into the defect (30 mm) of the goat tibias. The X-ray films were gathered and analyzed at the different time points. At 24 weeks post-operation, tissue engineering bones implanted were analyzed by histology and Micro-CT. Results show that the capacity of osteogenesis in experimental group was higher than that of control group by X-ray, histological, and micro-CT analysis (p < 0.05). According to the study, we found that repair for segmental bone defects of tissue engineering bone cultured by dynamic perfusion culture bioreactor outweigh those cultured in static state. And we can conclude that this technology of tissue engineering bone will become a clinical method to the segmental bone-defects repair in the future. (c) 2009 Wiley Periodicals, Inc.