Nicotinic receptors concentrated in the subsynaptic membrane do not contribute significantly to synaptic currents at an embryonic synapse in the chicken ciliary ganglion.

Rapid synaptic transmission at the calyciform synapse in the embryonic chicken ciliary ganglion is mediated by two classes of nicotinic receptors: those containing alpha3 subunits [alpha3-nicotinic ACh receptors (nAChRs)] and those containing alpha7 subunits (alpha7-nAChRs). alpha3-nAChRs and alpha7-nAChRs are differentially distributed on the cell surface; alpha3-nAChRs are concentrated at postsynaptic densities, whereas both alpha7-nAChRs and alpha3-nAChRs are found extrasynaptically on somatic spines. I explored the contribution of alpha3-nAChRs and alpha7-nAChRs to uniquantal responses, measured as mEPSCs, or as evoked responses under low release probability conditions. The contribution that each nAChR makes to uniquantal response shape was determined by blocking one nAChR type; pharmacologically isolated alpha7-nAChR responses were kinetically fast (rise time, 0.32 +/- 0.02 ms; decay time, 1.66 +/- 0.18 ms; mean +/- SD; n = 6 cells), whereas pharmacologically isolated alpha3-nAChR responses were slow (rise time, 1.28 +/- 0.35 ms; decay time, 6.71 +/- 1.46 ms; n = 8 cells). In the absence of antagonists, most cells (11 of 14) showed heterogeneity in the kinetics of uniquantal responses, with approximately 25% of events exhibiting fast, alpha7-nAChR-like kinetics and approximately 75% of events exhibiting the kinetics expected of coactivation of alpha7-nAChRs and alpha3-nAChRs. Cells rarely showed significant numbers of uniquantal responses with slow, alpha3-nAChR-like kinetics, which was unexpected given that alpha3-nAChRs alone are concentrated at postsynaptic densities. The only site where ACh quanta can activate both alpha3-nAChRs and alpha7-nAChRs readily is on the somatic spines, where alpha7-nAChRs and alpha3-nAChRs are present extrasynaptically. At the calyciform synapse, rapid synaptic transmission is mediated apparently without participation of ionotropic receptors concentrated at postsynaptic densities.