Literature DB >> 19319598

Expression, purification and molecular structure modeling of thioredoxin (Trx) and thioredoxin reductase (TrxR) from Acidithiobacillus ferrooxidans.

Yiping Wang1, Xiaojian Zhang, Qing Liu, Chenbing Ai, Hongyu Mo, Jia Zeng.   

Abstract

The thioredoxin system consists of thioredoxin (Trx), thioredoxin reductase (TrxR) and NADPH, which plays several key roles in maintaining the redox environment of the cell. In Acidithiobacillus ferrooxidans, thioredoxin system may play important functions in the activity regulation of periplasmic proteins and energy metabolism. Here, we cloned thioredoxin (trx) and thioredoxin reductase (trxR) genes from Acidithiobacillus ferrooxidans, and expressed the genes in Escherichia coli. His-Trx and His-TrxR were purified to homogeneity with one-step Ni-NTA affinity column chromatography. Site-directed mutagenesis results confirmed that Cys33, Cys36 of thioredoxin, and Cys142, Cys145 of thioredoxin reductase were active-site residues.

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Year:  2009        PMID： 19319598     DOI： 10.1007/s00284-009-9390-2

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  20 in total

1.  Molecular cloning and characterization of a mitochondrial selenocysteine-containing thioredoxin reductase from rat liver.

Authors:  S R Lee; J R Kim; K S Kwon; H W Yoon; R L Levine; A Ginsburg; S G Rhee
Journal:  J Biol Chem       Date:  1999-02-19       Impact factor: 5.157

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Journal:  Proc Natl Acad Sci U S A       Date:  1975-06       Impact factor: 11.205

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Authors:  C H Williams
Journal:  FASEB J       Date:  1995-10       Impact factor: 5.191

5.  Contributions of cysteine residues in Zn2 to zinc fingers and thiol-disulfide oxidoreductase activities of chaperone DnaJ.

Authors:  Yuan-yuan Shi; Wei Tang; Shu-feng Hao; Chih-Chen Wang
Journal:  Biochemistry       Date:  2005-02-08       Impact factor: 3.162

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Authors:  E S Arnér; A Holmgren
Journal:  Eur J Biochem       Date:  2000-10

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Authors:  Ju-A Kim; Sunjoo Park; Kangwha Kim; Sue Goo Rhee; Sang Won Kang
Journal:  Anal Biochem       Date:  2005-03-15       Impact factor: 3.365

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Authors:  M Russel; P Model
Journal:  J Biol Chem       Date:  1988-06-25       Impact factor: 5.157

Review 9.  Thioredoxin structure and mechanism: conformational changes on oxidation of the active-site sulfhydryls to a disulfide.

Authors:  A Holmgren
Journal:  Structure       Date:  1995-03-15       Impact factor: 5.006

10.  Characteristics and adaptability of iron- and sulfur-oxidizing microorganisms used for the recovery of metals from minerals and their concentrates.

Authors:  Douglas E Rawlings
Journal:  Microb Cell Fact       Date:  2005-05-06       Impact factor: 5.328
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  1 in total

1.  Heat and phosphate starvation effects on the proteome, morphology and chemical composition of the biomining bacteria Acidithiobacillus ferrooxidans.

Authors:  Daniela A Ribeiro; Danilo A Maretto; Fábio C S Nogueira; Márcio J Silva; Francisco A P Campos; Gilberto B Domont; Ronei J Poppi; Laura M M Ottoboni
Journal:  World J Microbiol Biotechnol       Date:  2010-11-04       Impact factor: 3.312
  1 in total

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