AIMS: The production of a homogenous population of human cardiomyocytes that can be expanded in vitro may facilitate development of replacement tissue lost as a result of cardiac disease and injury. MATERIALS AND METHODS: We evaluated the utility of activated leukocyte cell-adhesion molecule, CD166 (ALCAM) expression as a marker for isolating cardiomyocytes from differentiating cultures of human embryonic stem cells (hESCs). Using RT-qPCR, immunohistochemistry and DNA methylation studies, we evaluated the developmental age of hESC-derived cardiomyocytes. RESULTS AND CONCLUSIONS: We demonstrate that cardiomyocytes derived from hESC cultures express ALCAM and that this surface antigen can be used to select a population of differentiated cells that are enriched for cardiomyocytes. Expression of contractile proteins and ion channels, and DNA methylation patterns, suggest that ALCAM-enriched cardiomyocytes have an embryonic phenotype. Selected cardiomyocyte populations survive sorting, adhere to collagen-coated tissue culture plastic and proliferate in short-term culture. Long-term in vitro survival of cardiomyocytes was achieved by culturing cells in 3D aggregates.
AIMS: The production of a homogenous population of human cardiomyocytes that can be expanded in vitro may facilitate development of replacement tissue lost as a result of cardiac disease and injury. MATERIALS AND METHODS: We evaluated the utility of activated leukocyte cell-adhesion molecule, CD166 (ALCAM) expression as a marker for isolating cardiomyocytes from differentiating cultures of human embryonic stem cells (hESCs). Using RT-qPCR, immunohistochemistry and DNA methylation studies, we evaluated the developmental age of hESC-derived cardiomyocytes. RESULTS AND CONCLUSIONS: We demonstrate that cardiomyocytes derived from hESC cultures express ALCAM and that this surface antigen can be used to select a population of differentiated cells that are enriched for cardiomyocytes. Expression of contractile proteins and ion channels, and DNA methylation patterns, suggest that ALCAM-enriched cardiomyocytes have an embryonic phenotype. Selected cardiomyocyte populations survive sorting, adhere to collagen-coated tissue culture plastic and proliferate in short-term culture. Long-term in vitro survival of cardiomyocytes was achieved by culturing cells in 3D aggregates.
Authors: Pieterjan Dierickx; Pieter A Doevendans; Niels Geijsen; Linda W van Laake Journal: J Cardiovasc Transl Res Date: 2012-07-18 Impact factor: 4.132
Authors: Kornélia Szebényi; Adrienn Péntek; Zsuzsa Erdei; György Várady; Tamás I Orbán; Balázs Sarkadi; Ágota Apáti Journal: Tissue Eng Part C Methods Date: 2015-01 Impact factor: 3.056
Authors: Bo Lin; Jong Kim; Yanxin Li; Haiying Pan; Xonia Carvajal-Vergara; Guy Salama; Tao Cheng; Yong Li; Cecilia W Lo; Lei Yang Journal: Cardiovasc Res Date: 2012-06-06 Impact factor: 10.787
Authors: Adriana Blazeski; Renjun Zhu; David W Hunter; Seth H Weinberg; Kenneth R Boheler; Elias T Zambidis; Leslie Tung Journal: Prog Biophys Mol Biol Date: 2012-08-07 Impact factor: 3.667