Generation of recombinant baculovirus via liposome-mediated transfection.

Baculovirus expression vectors have become a popular method of producing recombinant proteins. Production of recombinant virus requires the transfection of both the native viral DNA and a transfer plasmid into insect cells where recombination takes place. While several methods of transfecting insect cells exist, we have found liposome-mediated transfection to be highly efficient. Here we detail the protocols and medium needed for efficient, simple transfection of Spodoptera frugiperda cells.