Shun-ichiro Asahara1, Tomokazu Matsuda, Yoshiaki Kido, Masato Kasuga. 1. Department of Internal Medicine, Division of Diabetes, Metabolism, and Endocrinology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
Abstract
AIM: To study the changes in gene expression by pancreatic beta cells under insulin resistance conditions. METHOD: An exhaustive gene expression analysis was performed, using isolated pancreatic islets of obese diabetic model Lepr(-/-) mice. Overexpression of cyclin D2 was induced in cells from the pancreatic beta cell line, namely, INS-1. RESULTS: Through a gene expression analysis using islets isolated from db/db mice, we found a significant increase in the expression of ribosome-related molecules. In addition, increased expression of cyclin D2 was found at certain protein levels. As INS-1 cells were induced to overexpress cyclin D2, we found an increase in the expression of ribosome-related molecules. Concurrently, an increase in the expression of endoplasmic reticulum stress (ER stress)-related molecules was also found. CONCLUSION: In cases of pancreatic beta cell hyperplasia associated with insulin resistance, ribosomal biogenesis is increased, and ER stress is induced.
AIM: To study the changes in gene expression by pancreatic beta cells under insulin resistance conditions. METHOD: An exhaustive gene expression analysis was performed, using isolated pancreatic islets of obese diabetic model Lepr(-/-) mice. Overexpression of cyclin D2 was induced in cells from the pancreatic beta cell line, namely, INS-1. RESULTS: Through a gene expression analysis using islets isolated from db/db mice, we found a significant increase in the expression of ribosome-related molecules. In addition, increased expression of cyclin D2 was found at certain protein levels. As INS-1 cells were induced to overexpress cyclin D2, we found an increase in the expression of ribosome-related molecules. Concurrently, an increase in the expression of endoplasmic reticulum stress (ER stress)-related molecules was also found. CONCLUSION: In cases of pancreatic beta cell hyperplasia associated with insulin resistance, ribosomal biogenesis is increased, and ER stress is induced.
Authors: Prasoon Agarwal; Navdeep Brar; Taylor S Morriseau; Stephanie M Kereliuk; Mario A Fonseca; Laura K Cole; Aruni Jha; Bo Xiang; Kristin L Hunt; Nivedita Seshadri; Grant M Hatch; Christine A Doucette; Vernon W Dolinsky Journal: Endocrinology Date: 2019-08-01 Impact factor: 4.736