| Literature DB >> 19306836 |
Stephen J McConoughey1, Zoya V Niatsetskaya, Ralf Pasternack, Martin Hils, Rajiv R Ratan, Arthur J L Cooper.
Abstract
Aberrant transglutaminase (TG) activity has been implicated in the pathology of numerous diseases, including Huntington's disease and Alzheimer's disease. To fully characterize the role of TGs in these disorders, it is important that simple quantifiable assays be made available. The most commonly used assay currently employed requires significant time and a radioactive substrate. The assay described here uses a biotinylated substrate in conjunction with a dot blot apparatus to eliminate the use of radioactive substrates and allows relative transglutaminase activity to be measured simultaneously with minimal sample preparation in a large number of samples containing purified enzyme, cell extracts, or tissue homogenates.Entities:
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Year: 2009 PMID： 19306836 PMCID： PMC2845163 DOI： 10.1016/j.ab.2009.03.022
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365