OBJECTIVE: The purpose of this study was to investigate the interaction of SAA and SR-BI in remodeling of acute phase HDL (AP HDL). METHODS AND RESULTS: We used SAA and SR-BI adenoviral vector expression models to study the interaction between these entities. SR-BI processing of mouse AP HDL generated progressively smaller discreet HDL particles with distinct apolipoprotein compositions. SR-BI actions segregated apolipoproteins with the smallest particles containing only apoA-I. Larger remnants contained apoA-I, apoA-II, and SAA. Small apoA-I only particles failed to associate with preformed HDL, whereas larger remnants readily did. The presence of SAA on SR-BI-processed HDL particles propelled apoA-I to a small lipid-poor form and accelerated apoA-I catabolism. CONCLUSIONS: Data indicate that after core and surface HDL lipid perturbation by SR-BI, SAA propels apoA-I to a small lipid-poor form while accelerating HDL metabolism.
OBJECTIVE: The purpose of this study was to investigate the interaction of SAA and SR-BI in remodeling of acute phase HDL (AP HDL). METHODS AND RESULTS: We used SAA and SR-BI adenoviral vector expression models to study the interaction between these entities. SR-BI processing of mouse AP HDL generated progressively smaller discreet HDL particles with distinct apolipoprotein compositions. SR-BI actions segregated apolipoproteins with the smallest particles containing only apoA-I. Larger remnants contained apoA-I, apoA-II, and SAA. Small apoA-I only particles failed to associate with preformed HDL, whereas larger remnants readily did. The presence of SAA on SR-BI-processed HDL particles propelled apoA-I to a small lipid-poor form and accelerated apoA-I catabolism. CONCLUSIONS: Data indicate that after core and surface HDL lipid perturbation by SR-BI, SAA propels apoA-I to a small lipid-poor form while accelerating HDL metabolism.
Authors: M C de Beer; D M Durbin; L Cai; N Mirocha; A Jonas; N R Webb; F C de Beer; D R van Der Westhuyzen Journal: J Biol Chem Date: 2001-02-09 Impact factor: 5.157
Authors: Nancy R Webb; Lei Cai; Kristine S Ziemba; Jin Yu; Mark S Kindy; Deneys R van der Westhuyzen; Frederick C de Beer Journal: J Lipid Res Date: 2002-11 Impact factor: 5.922
Authors: D L Williams; M de La Llera-Moya; S T Thuahnai; S Lund-Katz; M A Connelly; S Azhar; G M Anantharamaiah; M C Phillips Journal: J Biol Chem Date: 2000-06-23 Impact factor: 5.157
Authors: G A Coetzee; A F Strachan; D R van der Westhuyzen; H C Hoppe; M S Jeenah; F C de Beer Journal: J Biol Chem Date: 1986-07-25 Impact factor: 5.157
Authors: Ji-Young Lee; Lorraine Lanningham-Foster; Elena Y Boudyguina; Thomas L Smith; Ellen R Young; Perry L Colvin; Michael J Thomas; John S Parks Journal: J Lipid Res Date: 2004-01-16 Impact factor: 5.922
Authors: Nancy R Webb; Maria C de Beer; Bela F Asztalos; Nathan Whitaker; Deneys R van der Westhuyzen; Frederick C de Beer Journal: J Lipid Res Date: 2004-06-21 Impact factor: 5.922
Authors: Joanne M Wroblewski; Anisa Jahangiri; Ailing Ji; Frederick C de Beer; Deneys R van der Westhuyzen; Nancy R Webb Journal: J Lipid Res Date: 2011-09-27 Impact factor: 5.922