Literature DB >> 19303871

Leishmania (Viannia) panamensis: an in vitro assay using the expression of GFP for screening of antileishmanial drug.

Rubén E Varela M1, Diana Lorena Muñoz, Sara M Robledo, Bala K Kolli, Sujoy Dutta, Kwang Poo Chang, Carlos Muskus.   

Abstract

Promastigotes of Leishmania (Viannia) panamensis were successfully transfected with p6.5-egfp to express green fluorescent protein. The transfectants remained infective to macrophages, providing an in vitro model for screening antileishmanial drugs. This was demonstrated by flow cytometry of macrophage-associated GFP after exposure of infected cultures to known antileishmanial drugs, i.e. amphotericin B and glucantime. Fluorescence of GFP diminished progressively from infected cells with increasing drug concentrations used in both cases. The availability of this fluorescent assay for infection of macrophages by L. (V.) panamensis facilitates drug discovery program for the Viannia species, which differ significantly from those of the Leishmania subgenus.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19303871      PMCID： PMC3640980          DOI： 10.1016/j.exppara.2009.02.012

Source DB:  PubMed          Journal:  Exp Parasitol        ISSN： 0014-4894            Impact factor:   2.011


  42 in total

1.  Episomal expression of specific sense and antisense mRNAs in Leishmania amazonensis: modulation of gp63 level in promastigotes and their infection of macrophages in vitro.

Authors:  D Q Chen; B K Kolli; N Yadava; H G Lu; A Gilman-Sachs; D A Peterson; K P Chang
Journal:  Infect Immun       Date:  2000-01       Impact factor: 3.441

2.  Efficacy of second line drugs on antimonyl-resistant amastigotes of Leishmania infantum.

Authors:  D Sereno; P Holzmuller; J L Lemesre
Journal:  Acta Trop       Date:  2000-01-05       Impact factor: 3.112

3.  Statisical logic in the monitoring of reactions to therapeutic drugs.

Authors:  D J Finney
Journal:  Methods Inf Med       Date:  1971-10       Impact factor: 2.176

4.  Isolation of a cDNA encoding CD33, a differentiation antigen of myeloid progenitor cells.

Authors:  D Simmons; B Seed
Journal:  J Immunol       Date:  1988-10-15       Impact factor: 5.422

5.  Establishment and characterization of a human histiocytic lymphoma cell line (U-937).

Authors:  C Sundström; K Nilsson
Journal:  Int J Cancer       Date:  1976-05-15       Impact factor: 7.396

6.  Loss of the GP46/M-2 surface membrane glycoprotein gene family in the Leishmania braziliensis complex.

Authors:  D McMahon-Pratt; Y Traub-Cseko; K L Lohman; D D Rogers; S M Beverley
Journal:  Mol Biochem Parasitol       Date:  1992-01       Impact factor: 1.759

7.  Leishmania spp.: delta-aminolevulinate-inducible neogenesis of porphyria by genetic complementation of incomplete heme biosynthesis pathway.

Authors:  Sujoy Dutta; Kazumichi Furuyama; Shigeru Sassa; Kwang-Poo Chang
Journal:  Exp Parasitol       Date:  2007-12-03       Impact factor: 2.011

8.  The 63-kilobase circular amplicon of tunicamycin-resistant Leishmania amazonensis contains a functional N-acetylglucosamine-1-phosphate transferase gene that can be used as a dominant selectable marker in transfection.

Authors:  X Liu; K P Chang
Journal:  Mol Cell Biol       Date:  1992-09       Impact factor: 4.272

Review 9.  Does the Leishmania major paradigm of pathogenesis and protection hold for New World cutaneous leishmaniases or the visceral disease?

Authors:  Diane McMahon-Pratt; James Alexander
Journal:  Immunol Rev       Date:  2004-10       Impact factor: 12.988

10.  Applications of recombinant Leishmania amazonensis expressing egfp or the beta-galactosidase gene for drug screening and histopathological analysis.

Authors:  Takahiro Okuno; Yasuyuki Goto; Yoshitsugu Matsumoto; Haruki Otsuka; Yasunobu Matsumoto
Journal:  Exp Anim       Date:  2003-04
View more
  20 in total

1.  Flow cytometric screening for anti-leishmanials in a human macrophage cell line.

Authors:  Sanjay R Mehta; Xing-Quan Zhang; Roberto Badaro; Celsa Spina; John Day; Kwang-Poo Chang; Robert T Schooley
Journal:  Exp Parasitol       Date:  2010-06-09       Impact factor: 2.011

2.  Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies.

Authors:  Andrés Montoya; Alejandro Daza; Diana Muñoz; Karina Ríos; Viviana Taylor; David Cedeño; Iván D Vélez; Fernando Echeverri; Sara M Robledo
Journal:  Antimicrob Agents Chemother       Date:  2015-07-13       Impact factor: 5.191

3.  Local Delivery of the Toll-Like Receptor 9 Ligand CpG Downregulates Host Immune and Inflammatory Responses, Ameliorating Established Leishmania (Viannia) panamensis Chronic Infection.

Authors:  Allison K Ehrlich; Olga L Fernández; Daniel Rodriguez-Pinto; Tiago M Castilho; Maria J Corral Caridad; Karen Goldsmith-Pestana; Nancy Gore Saravia; Diane McMahon-Pratt
Journal:  Infect Immun       Date:  2017-02-23       Impact factor: 3.441

4.  Selective fusion of azurophilic granules with Leishmania-containing phagosomes in human neutrophils.

Authors:  Faustino Mollinedo; Hans Janssen; Janis de la Iglesia-Vicente; Janny A Villa-Pulgarin; Jero Calafat
Journal:  J Biol Chem       Date:  2010-08-26       Impact factor: 5.157

5.  The immunotherapeutic role of regulatory T cells in Leishmania (Viannia) panamensis infection.

Authors:  Allison Ehrlich; Tiago Moreno Castilho; Karen Goldsmith-Pestana; Wook-Jin Chae; Alfred L M Bothwell; Tim Sparwasser; Diane McMahon-Pratt
Journal:  J Immunol       Date:  2014-08-06       Impact factor: 5.422

6.  Protein network prediction and topological analysis in Leishmania major as a tool for drug target selection.

Authors:  Andrés F Flórez; Daeui Park; Jong Bhak; Byoung-Chul Kim; Allan Kuchinsky; John H Morris; Jairo Espinosa; Carlos Muskus
Journal:  BMC Bioinformatics       Date:  2010-09-27       Impact factor: 3.169

7.  An alternative in vitro drug screening test using Leishmania amazonensis transfected with red fluorescent protein.

Authors:  Marcele N Rocha; Célia M Corrêa; Maria N Melo; Stephen M Beverley; Olindo Assis Martins-Filho; Ana Paula Madureira; Rodrigo P Soares
Journal:  Diagn Microbiol Infect Dis       Date:  2013-01-10       Impact factor: 2.803

8.  Intracellular targeting specificity of novel phthalocyanines assessed in a host-parasite model for developing potential photodynamic medicine.

Authors:  Sujoy Dutta; Benson G Ongarora; Hairong Li; Maria da Graca H Vicente; Bala K Kolli; Kwang Poo Chang
Journal:  PLoS One       Date:  2011-06-06       Impact factor: 3.240

9.  Antiparasitic bromotyrosine derivatives from the marine sponge Verongula rigida.

Authors:  Elkin Galeano; Olivier P Thomas; Sara Robledo; Diana Munoz; Alejandro Martinez
Journal:  Mar Drugs       Date:  2011-10-14       Impact factor: 6.085

10.  Use of in vivo and in vitro systems to select Leishmania amazonensis expressing green fluorescent protein.

Authors:  Solange dos Santos Costa; Marjorie de Assis Golim; Bartira Rossi-Bergmann; Fabio Trindade Maranhão Costa; Selma Giorgio
Journal:  Korean J Parasitol       Date:  2011-12-16       Impact factor: 1.341
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.