| Literature DB >> 19303392 |
Yee-Guide Yeung1, E Richard Stanley.
Abstract
Available protocols for stripping antibodies from immunoblots involve the use of sodium dodecyl sulfate (SDS) or low-pH buffers. SDS was shown to remove transferred proteins from membranes, and low-pH buffer was shown to inefficiently strip off antibodies. A solution containing 6M guanidine hydrochloride, 0.2% nondenaturing detergent, and a reducing agent can rapidly strip off tightly bound antibodies from aged polyvinylidene fluoride (PVDF) immunoblots at room temperature without removing significant amounts of transferred protein.Entities:
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Year: 2009 PMID： 19303392 PMCID： PMC2679975 DOI： 10.1016/j.ab.2009.03.017
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365