| Literature DB >> 19302791 |
Joachim Zettler1, Vivien Schütz, Henning D Mootz.
Abstract
We have studied the naturally split alpha subunit of the DNA polymerase III (DnaE) intein from Nostoc punctiforme PCC73102 (Npu) using purified proteins and determined an apparent first-order rate constant of (1.1+/-0.2)x10(-2) s(-1) at 37 degrees C. This represents the highest rate reported for the protein trans-splicing reaction so far (t(1/2) of approximately 60s). Furthermore, the reaction was very robust and high-yielding with respect to different extein sequences, temperatures from 6 to 37 degrees C, and the presence of up to 6 M urea. Given these outstanding properties, the Npu DnaE intein appears to be the intein of choice for many applications in protein and cellular chemistry.Entities:
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Year: 2009 PMID： 19302791 DOI： 10.1016/j.febslet.2009.02.003
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124