Literature DB >> 19301623

Genotyping of IRGM tetranucleotide promoter oligorepeats by fluorescence resonance energy transfer.

Christopher D Intemann1, Thorsten Thye, Jürgen Sievertsen, Ellis Owusu-Dabo, Rolf D Horstmann, Christian G Meyer.   

Abstract

Fluorescence resonance energy transfer (FRET) genotyping has been well established for the rapid assessment of single nucleotide polymorphisms (SNPs) and deletions. A design is presented that allows the typing of short tandem oligo repeat sequences using the LightTyper/LightCycler system. The protocol was evaluated and applied to the typing of a tetranucleotide promoter repeat of the human gene encoding the immunity-related GTPase family, M (IRGM) molecule in >2000 individuals from Ghana, West Africa.

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Year:  2009        PMID： 19301623     DOI： 10.2144/000113045

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  4 in total

Review 1.  Pharmacogenomics of CYP3A: considerations for HIV treatment.

Authors:  Sukhwinder S Lakhman; Qing Ma; Gene D Morse
Journal:  Pharmacogenomics       Date:  2009-08       Impact factor: 2.533

2.  Autophagy gene variant IRGM -261T contributes to protection from tuberculosis caused by Mycobacterium tuberculosis but not by M. africanum strains.

Authors:  Christopher D Intemann; Thorsten Thye; Stefan Niemann; Edmund N L Browne; Margaret Amanua Chinbuah; Anthony Enimil; John Gyapong; Ivy Osei; Ellis Owusu-Dabo; Susanne Helm; Sabine Rüsch-Gerdes; Rolf D Horstmann; Christian G Meyer
Journal:  PLoS Pathog       Date:  2009-09-11       Impact factor: 6.823

3.  Rapid and inexpensive body fluid identification by RNA profiling-based multiplex High Resolution Melt (HRM) analysis.

Authors:  Erin K Hanson; Jack Ballantyne
Journal:  F1000Res       Date:  2013-12-20

4.  Independent and population-specific association of risk variants at the IRGM locus with Crohn's disease.

Authors:  Natalie J Prescott; Katherine M Dominy; Michiaki Kubo; Cathryn M Lewis; Sheila A Fisher; Richard Redon; Ni Huang; Barbara E Stranger; Katarzyna Blaszczyk; Barry Hudspith; Gareth Parkes; Naoya Hosono; Keiko Yamazaki; Clive M Onnie; Alastair Forbes; Emmanouil T Dermitzakis; Yusuke Nakamura; John C Mansfield; Jeremy Sanderson; Matthew E Hurles; Roland G Roberts; Christopher G Mathew
Journal:  Hum Mol Genet       Date:  2010-01-27       Impact factor: 6.150
  4 in total

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