Literature DB >> 19300826

Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase.

Rakesh N Veedu1, Birte Vester, Jesper Wengel.   

Abstract

Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and LNA-5-methyl-CTP, were investigated as substrates for KOD DNA polymerase. The results reveal that KOD DNA polymerase is an efficient catalyst for template directed synthesis of DNA oligonucleotide duplexes containing a large number of LNA nucleotides by primer extension reactions. Furthermore, KOD DNA polymerase is shown to be suitable for the PCR amplification of an LNA-modified DNA duplex.

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Year:  2009        PMID： 19300826     DOI： 10.1039/b819946a

Source DB:  PubMed          Journal:  Org Biomol Chem        ISSN： 1477-0520            Impact factor:   3.876


  21 in total

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