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Literature DB >> 1929875

Serial propagation of porcine enteric calicivirus in a continuous cell line. Effect of medium supplementation with intestinal contents or enzymes.

A V Parwani¹, W T Flynn, K L Gadfield, L J Saif.

Abstract

A porcine enteric calicivirus (PEC) was adapted to serial propagation in a continuous porcine kidney cell line (LLC-PK) by inclusion of an intestinal contents preparation (ICP), derived from uninfected gnotobiotic pigs, in the maintenance medium. Infected cells were detected by immunofluorescent staining of cell monolayers. Attempts to propagate PEC in the presence of various intestinal enzymes of porcine origin, bacterial protease, bovine ICP, avian ICP or without medium supplementation were unsuccessful.

Entities: Species

Mesh：

Substances：
Culture Media

Year: 1991 PMID： 1929875 DOI： 10.1007/bf01310954

Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574

16 in total

1. Cell culture propagation of porcine rotavirus (reovirus-like agent).

Authors: K W Theil; E H Bohl; A G Agnes
Journal: Am J Vet Res Date: 1977-11 Impact factor: 1.156

2. Porcine rotaviral infection of cell culture: effects of certain enzymes.

Authors: K W Theil; E H Bohl
Journal: Am J Vet Res Date: 1980-01 Impact factor: 1.156

3. Serial propagation of porcine enteric calicivirus-like virus in primary porcine kidney cell cultures.

Authors: W T Flynn; L J Saif
Journal: J Clin Microbiol Date: 1988-02 Impact factor: 5.948

Review 4. Caliciviruses. Brief review.

Authors: M J Studdert
Journal: Arch Virol Date: 1978 Impact factor: 2.574

5. Characterization of a new calicivirus isolated from feces of a dog.

Authors: F L Schaffer; M E Soergel; J W Black; D E Skilling; A W Smith; W D Cubitt
Journal: Arch Virol Date: 1985 Impact factor: 2.574

6. Pathogenesis of porcine enteric calicivirus-like virus in four-day-old gnotobiotic pigs.

Authors: W T Flynn; L J Saif; P D Moorhead
Journal: Am J Vet Res Date: 1988-06 Impact factor: 1.156

7. Biochemical characterization of porcine enteric calicivirus: analysis of structural and nonstructural viral proteins.

Authors: A V Parwani; L J Saif; S Y Kang
Journal: Arch Virol Date: 1990 Impact factor: 2.574

8. Rotavirus-like, calicivirus-like, and 23-nm virus-like particles associated with diarrhea in young pigs.

Authors: L J Saif; E H Bohl; K W Theil; R F Cross; J A House
Journal: J Clin Microbiol Date: 1980-07 Impact factor: 5.948

9. Propagation and preliminary characterization of a chicken candidate calicivirus.

Authors: W D Cubitt; A D Barrett
Journal: J Gen Virol Date: 1985-07 Impact factor: 3.891

10. Human calicivirus-associated diarrhea in children attending day care centers.

Authors: D O Matson; M K Estes; R I Glass; A V Bartlett; M Penaranda; E Calomeni; T Tanaka; S Nakata; S Chiba
Journal: J Infect Dis Date: 1989-01 Impact factor: 5.226

35 in total

Review 1. Porcine enteric caliciviruses: genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology.

Authors: Qiu-Hong Wang; Veronica Costantini; Linda J Saif
Journal: Vaccine Date: 2006-12-29 Impact factor: 3.641

Serial propagation of porcine enteric calicivirus in a continuous cell line. Effect of medium supplementation with intestinal contents or enzymes.

1. Cell culture propagation of porcine rotavirus (reovirus-like agent).

2. Porcine rotaviral infection of cell culture: effects of certain enzymes.

3. Serial propagation of porcine enteric calicivirus-like virus in primary porcine kidney cell cultures.

Review 4. Caliciviruses. Brief review.

5. Characterization of a new calicivirus isolated from feces of a dog.

6. Pathogenesis of porcine enteric calicivirus-like virus in four-day-old gnotobiotic pigs.

7. Biochemical characterization of porcine enteric calicivirus: analysis of structural and nonstructural viral proteins.

8. Rotavirus-like, calicivirus-like, and 23-nm virus-like particles associated with diarrhea in young pigs.

9. Propagation and preliminary characterization of a chicken candidate calicivirus.

10. Human calicivirus-associated diarrhea in children attending day care centers.

Review 1. Porcine enteric caliciviruses: genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology.

2. Molecular characterization of a bovine enteric calicivirus: relationship to the Norwalk-like viruses.

3. Model systems for the study of human norovirus Biology.

Review 4. Comprehensive review of human sapoviruses.

5. Stability of and attachment to lettuce by a culturable porcine sapovirus surrogate for human caliciviruses.

6. Reverse genetics system for porcine enteric calicivirus, a prototype sapovirus in the Caliciviridae.

7. Genetic diversity and recombination of porcine sapoviruses.

8. Characterization of a rhesus monkey calicivirus representing a new genus of Caliciviridae.

9. Role of cholesterol pathways in norovirus replication.

Review 10. Norovirus immunology: Of mice and mechanisms.