| Literature DB >> 19295654 |
Santosh C Jagtap1, Chandrakant B Jagtap, Pradeep Kumar, R B Srivastava.
Abstract
A multiplex colony PCR assay was developed for the detection of 5 genes encoding Bacillus sphaericus mosquito larvicidal toxins, namely binA, binB, mtx1, mtx2, and mtx3. Primers designed for these 5 genes yielded specific PCR amplicons of the expected size from type cultures of B. sphaericus. This method of detecting multiple toxin genes by colony PCR in a single tube reaction is a simple, rapid, and economical technique for identification of highly toxic environmental B. sphaericus isolates.Entities:
Mesh：
Substances：
Year: 2009 PMID： 19295654 DOI： 10.1139/w08-113
Source DB: PubMed Journal: Can J Microbiol ISSN： 0008-4166 Impact factor: 2.419