Sitaram Parvataneni1, Babu Gonipeta, Robert J Tempelman, Venu Gangur. 1. Food Allergy and Immunology Laboratory, Department of Food Science and Human Nutrition, Nutritional Immunology Program, Michigan State University, East Lansing, Michigan 48823, USA.
Abstract
BACKGROUND: Cashew nut allergy is an emerging food allergy with a high risk of systemic anaphylaxis. Currently, an adjuvant-free animal model to study cashew nut allergy is not available. METHODS: BALB/c mice were exposed to cashew nut protein using a transdermal sensitization protocol that does not use adjuvant. Systemic IgE antibody response, systemic anaphylaxis to oral challenge and allergen-driven, spleen-cell, type-2 cytokine responses were studied. RESULTS: Transdermal exposure to cashew nut resulted in a significant dose-dependent allergic response. Oral challenge of sensitized mice with cashew resulted in severe signs of systemic anaphylaxis and a significant hypothermia. Spleen cell culture with cashew nut protein resulted in allergen-driven IL-4, IL-5 and IL-13 responses only in sensitized but not in saline control mice. CONCLUSIONS: These data demonstrate that (i) transdermal exposure to cashew nut protein elicits a robust IgE response leading to clinical sensitization of mice for systemic anaphylaxis to oral cashew nut challenge; (ii) cashew nut is a potent activator of type-2 cytokines, thus explaining the mechanism of cashew allergy, and (iii) this mouse model may be useful for further basic and preclinical studies on cashew nut allergy. Copyright (C) 2009 S. Karger AG, Basel.
BACKGROUND:Cashew nut allergy is an emerging food allergy with a high risk of systemic anaphylaxis. Currently, an adjuvant-free animal model to study cashew nut allergy is not available. METHODS: BALB/c mice were exposed to cashew nut protein using a transdermal sensitization protocol that does not use adjuvant. Systemic IgE antibody response, systemic anaphylaxis to oral challenge and allergen-driven, spleen-cell, type-2 cytokine responses were studied. RESULTS: Transdermal exposure to cashew nut resulted in a significant dose-dependent allergic response. Oral challenge of sensitized mice with cashew resulted in severe signs of systemic anaphylaxis and a significant hypothermia. Spleen cell culture with cashew nut protein resulted in allergen-driven IL-4, IL-5 and IL-13 responses only in sensitized but not in saline control mice. CONCLUSIONS: These data demonstrate that (i) transdermal exposure to cashew nut protein elicits a robust IgE response leading to clinical sensitization of mice for systemic anaphylaxis to oral cashew nut challenge; (ii) cashew nut is a potent activator of type-2 cytokines, thus explaining the mechanism of cashewallergy, and (iii) this mouse model may be useful for further basic and preclinical studies on cashew nut allergy. Copyright (C) 2009 S. Karger AG, Basel.
Authors: Ovidiu Ivanciuc; Steven M Gendel; Trevor D Power; Catherine H Schein; Werner Braun Journal: Regul Toxicol Pharmacol Date: 2011-03-21 Impact factor: 3.271
Authors: Michele S Barros; Eliane Gomes; Daniele I Gueroni; Anderson D Ramos; Luciana Mirotti; Esther Florsheim; Bruna Bizzarro; Ciro N R Lino; Ceres Maciel; Adriana Lino-Dos-Santos-Franco; Wothan Tavares-de-Lima; Margareth L Capurro; Momtchilo Russo; Anderson Sá-Nunes Journal: PLoS One Date: 2016-05-20 Impact factor: 3.240