PURPOSE: Programmed death-1 (PD-1) ligation downregulates active lymphocyte responses. The authors tested whether PD-1 or its ligands are expressed in the posterior segment during active intraocular inflammation. METHODS: Experimental autoimmune uveitis (EAU) was induced using interphotoreceptor retinoid-binding protein (IRBP 161-180). Ocular inflammation was evaluated by histology and expression of PD-1 ligand tested by immunohistochemistry. PD-1 expression was evaluated by immunohistochemistry, RT-PCR, and Western immunoblotting. RESULTS: Using immunohistochemistry, PD-1, but not its ligands, was constitutively expressed in retinal neurons of naive mouse retina. Both PD-1 and its ligands were observed, as expected, in sites of active inflammation. CONCLUSIONS: PD-1 and its ligands were expressed in sites of active inflammation, in accordance with many other models of inflammatory disease. Surprisingly, PD-1, not previously described outside the immune system, was constitutively expressed in retinal neurons, raising the possibility that PD-1 signaling may be important for neuronal function in the absence of an inflammatory insult.
PURPOSE: Programmed death-1 (PD-1) ligation downregulates active lymphocyte responses. The authors tested whether PD-1 or its ligands are expressed in the posterior segment during active intraocular inflammation. METHODS: Experimental autoimmune uveitis (EAU) was induced using interphotoreceptor retinoid-binding protein (IRBP 161-180). Ocular inflammation was evaluated by histology and expression of PD-1 ligand tested by immunohistochemistry. PD-1 expression was evaluated by immunohistochemistry, RT-PCR, and Western immunoblotting. RESULTS: Using immunohistochemistry, PD-1, but not its ligands, was constitutively expressed in retinal neurons of naive mouse retina. Both PD-1 and its ligands were observed, as expected, in sites of active inflammation. CONCLUSIONS: PD-1 and its ligands were expressed in sites of active inflammation, in accordance with many other models of inflammatory disease. Surprisingly, PD-1, not previously described outside the immune system, was constitutively expressed in retinal neurons, raising the possibility that PD-1 signaling may be important for neuronal function in the absence of an inflammatory insult.
Authors: Wei Wang; Ann Chan; Yu Qin; Jacky M K Kwong; Joseph Caprioli; Ralph Levinson; Ling Chen; Lynn K Gordon Journal: Exp Eye Res Date: 2015-08-13 Impact factor: 3.467
Authors: Caroline W Sham; Ann M Chan; Jacky M K Kwong; Joseph Caprioli; Steven Nusinowitz; Bryan Chen; Janice G Lee; Nishant M Gandhi; Loise M Francisco; Arlene H Sharpe; Ling Chen; Jonathan Braun; Lynn K Gordon Journal: J Neuroophthalmol Date: 2012-09 Impact factor: 3.042
Authors: Ling Chen; Caroline W Sham; Ann M Chan; Loise M Francisco; Yin Wu; Sergey Mareninov; Arlene H Sharpe; Gordon J Freeman; Xian-Jie Yang; Jonathan Braun; Lynn K Gordon Journal: Invest Ophthalmol Vis Sci Date: 2009-05-06 Impact factor: 4.799