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Literature DB >> 192909

Polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody-containing agarose gel.

Abstract

Crossed immunoelectrophoresis was used to characterize herpes simplex virus type 1 (HSV-1) antigens produced by infected HEp-2 cells. We report on a method for analyzing the polypeptide content in individual antigen-antibody precipitates eluted from the second-dimensional agarose gel. Four glycoprotein antigens of HSV-1, Ag-8, Ag-11, Ag-6, and Ag-3, were isolated and analyzed for polypeptide content. The molecular weights of the polypeptides are presented.

Entities: Chemical Species

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Year: 1977 PMID： 192909 PMCID： PMC515691

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

16 in total

1. Some structural antigens of herpes simplex virus type 1.

Authors: K L Powell; D H Watson
Journal: J Gen Virol Date: 1975-11 Impact factor: 3.891

Review 2. Human herpersviruses I: a model for molecular organization and regulation of herpesviruses-a review.

Authors: B Roizman; G Hayward; R Jacob; S Wadsworth; N Frenkel; R W Honess; M Kozak
Journal: IARC Sci Publ Date: 1975

3. Herpes simplex virus-specific polypeptides studied by polyacrylamide gel electrophoresis of immune precipitates.

Authors: R W Honess; D H Watson
Journal: J Gen Virol Date: 1974-02 Impact factor: 3.891

4. A temperature-sensitive mutant of herpes simplex virus type 1 defective in the synthesis of the major capsid polypeptide.

Authors: D R Bone; R J Courtney
Journal: J Gen Virol Date: 1974-07 Impact factor: 3.891

5. Antigens specified by herpesviruses. 3. Viral-induced nuclear polypeptides.

Authors: R M McCombs
Journal: Virology Date: 1974-02 Impact factor: 3.616

6. Type-specific protein in herpes simplex virus envelope reacts with neutralising antibody.

Authors: K L Powell; A Buchan; C Sim; D H Watson
Journal: Nature Date: 1974-05-24 Impact factor: 49.962

7. Crossed immunoelectrophoretic characterization of Herpesvirus hominis type 1 and 2 antigens.

Authors: B F Vestergaard
Journal: Acta Pathol Microbiol Scand B Microbiol Immunol Date: 1973-12

8. Proteins specified by herpes simplex virus. VI. Viral proteins in the plasma membrane.

Authors: J W Heine; P G Spear; B Roizman
Journal: J Virol Date: 1972-03 Impact factor: 5.103

9. Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion.

Authors: P G Spear; B Roizman
Journal: J Virol Date: 1972-01 Impact factor: 5.103

10. Proteins specified by herpes simplex virus. XIII. Glycosylation of viral polypeptides.

Authors: R W Honess; B Roizman
Journal: J Virol Date: 1975-11 Impact factor: 5.103

12 in total

1. gA and gB glycoproteins of herpes simplex virus type 1: two forms of a single polypeptide.

Authors: R Eberle; R J Courtney
Journal: J Virol Date: 1980-12 Impact factor: 5.103

2. Quantitative immunoelectrophoretic analysis of human antibodies against herpes simplex virus antigens.

Authors: B F Vestergaard
Journal: Infect Immun Date: 1979-03 Impact factor: 3.441

3. Preparation and characterization of specific antisera to individual glycoprotein antigens comprising the major glycoprotein region of herpes simplex virus type 1.

Authors: R Eberle; R J Courtney
Journal: J Virol Date: 1980-09 Impact factor: 5.103

10. Immunological reactivity of herpes simplex virus 1 and 2 polypeptides electrophoretically separated and transferred to diazobenzyloxymethyl paper.

Authors: B Norrild; B Pedersen; B Roizman
Journal: Infect Immun Date: 1981-02 Impact factor: 3.441