Literature DB >> 192908

Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein.

J Luka, W Siegert, G Klein.   

Abstract

The Eptstein-Barr virus (EBV)-determined nuclear antigen (EBNA) was solubilized from isolate nuclei of two EBV-transformed cell lines- Raji and AW-Ramos, by high-salt treatment. Its DNA-binding properties were studied by DNA-cellulose chromatography and a 51Cr release complement fixation assay. EBNA binds to both double-stranded and single-stranded calf thymus DNA, showing a higher affinity to double-stranded DNA. There was no detectable difference in the DNA binding of EBNA prepared from Raji and AW-Ramos cells.

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Year:  1977        PMID： 192908      PMCID： PMC515679     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  34 in total

1.  Isolation and characterization of adenovirus type 12 DNA binding proteins.

Authors:  B Rosenwirth; K Shiroki; A J Levine; H Shimojo
Journal:  Virology       Date:  1975-09       Impact factor: 3.616

2.  A STUDY OF MALIGNANT TUMOURS IN NIGERIA BY SHORT-TERM TISSUE CULTURE.

Authors:  J V PULVERTAFT
Journal:  J Clin Pathol       Date:  1965-05       Impact factor: 3.411

3.  Function of simian virus 40 gene A in transforming infection.

Authors:  P Tegtmeyer
Journal:  J Virol       Date:  1975-03       Impact factor: 5.103

4.  An improved quantitative micro-complement fixation test.

Authors:  M Cikes
Journal:  J Immunol Methods       Date:  1975       Impact factor: 2.303

5.  Double-stranded DNA binding of adenovirus type 12 tumor antigen.

Authors:  K Raska; V Rubio; K K Biron
Journal:  FEBS Lett       Date:  1976-02-15       Impact factor: 4.124

6.  DNA infectivity and the induction of host DNA synthesis with temperature-sensitive mutants of simian virus 40.

Authors:  J Y Chou; R G Martin
Journal:  J Virol       Date:  1975-01       Impact factor: 5.103

7.  Simian virus 40 functions required for the establishment and maintenance of malignant transformation.

Authors:  R G Martin; J Y Chou
Journal:  J Virol       Date:  1975-03       Impact factor: 5.103

8.  Induction of Epstein-Barr virus-associated nuclear antigen during in vitro transformation of human lymphoid cells.

Authors:  W Leibold; T D Flanagan; J Menezes; G Klein
Journal:  J Natl Cancer Inst       Date:  1975-01       Impact factor: 13.506

9.  Role of simian virus 40 gene A function in maintenance of transformation.

Authors:  J S Brugge; J S Butel
Journal:  J Virol       Date:  1975-03       Impact factor: 5.103

10.  T antigen binds to simian virus 40 DNA at the origin of replication.

Authors:  S I Reed; J Ferguson; R W Davis; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1975-04       Impact factor: 11.205
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  18 in total

1.  No activation of new initiation points for deoxyribonucleic acid replication in BALB/c 3T3 cells transformed by Kirsten sarcoma virus.

Authors:  A Oppenheim; A T Horowitz
Journal:  Mol Cell Biol       Date:  1981-08       Impact factor: 4.272

2.  Epstein-Barr virus-specific RNA. III. Mapping of DNA encoding viral RNA in restringent infection.

Authors:  A L Powell; W King; E Kieff
Journal:  J Virol       Date:  1979-01       Impact factor: 5.103

3.  Use of isolated nuclei in the indirect fluorescent-antibody test for human cytomegalovirus infection: comparison with microneutralization, anticomplement, and conventional indirect fluorescent-antibody assays.

Authors:  S Stagno; D W Reynolds; R J Smith
Journal:  J Clin Microbiol       Date:  1978-05       Impact factor: 5.948

4.  Initiation points for cellular deoxyribonucleic acid replication in human lymphoid cells converted by Epstein-Barr virus.

Authors:  A Oppenheim; Z Shlomai; H Ben-Bassat
Journal:  Mol Cell Biol       Date:  1981-08       Impact factor: 4.272

5.  Identification with monoclonal antibodies of virus-specific DNA-binding proteins in the nuclei of cells infected with three serotypes of Marek's disease virus-related viruses.

Authors:  K Nakajima; K Ikuta; S Ueda; S Kato; K Hirai
Journal:  J Virol       Date:  1986-07       Impact factor: 5.103

6.  Purification of the Epstein-Barr virus-determined nuclear antigen from Epstein-Barr virus-transformed human lymphoid cell lines.

Authors:  J Luka; T Lindahl; G Klein
Journal:  J Virol       Date:  1978-09       Impact factor: 5.103

7.  Binding to chromosomes of herpes simplex-related antigens in biochemically transformed cells.

Authors:  S Kit; M Kurchak; W Wray; D R Dubbs
Journal:  Proc Natl Acad Sci U S A       Date:  1978-07       Impact factor: 11.205

8.  Purification and biochemical characterization of the Epstein-Barr virus-determined nuclear antigen and an associated protein with a 53,000-dalton subunit.

Authors:  J Luka; H Jörnvall; G Klein
Journal:  J Virol       Date:  1980-09       Impact factor: 5.103

9.  Stable expression in mouse cells of nuclear neoantigen after transfer of a 3.4-megadalton cloned fragment of Epstein-Barr virus DNA.

Authors:  W P Summers; E A Grogan; D Shedd; M Robert; C R Liu; G Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

10.  Induction of Epstein-Barr virus nuclear antigen and DNA synthesis in a human epithelial cell line after Epstein-Barr virus infection.

Authors:  H Ben-Bassat; S Mitrani-Rosenbaum; N Goldblum
Journal:  J Virol       Date:  1982-02       Impact factor: 5.103
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