Literature DB >> 19288134

Development of a real-time PCR for the differentiation of the G1 and G2/G3 genotypes of Echinococcus granulosus.

Maria Paola Maurelli1, Laura Rinaldi, Federico Capuano, Anna Giannina Perugini, Giuseppe Cringoli.   

Abstract

The present study was aimed at developing a SYBR Green-based real-time polymerase chain reaction (PCR) assay for a rapid differentiation of the genotype G1 from the cluster of genotypes G2/G3 of Echinococcus granulosus, using as marker the 12S mtDNA gene. Eleven hydatid cysts from water buffaloes and 19 from cattle were used. Fourteen samples (identified as G1 using sequencing) showed a mean melting temperature (T (m)) of 76.4 degrees C and 16 samples (identified as G2/G3 using sequencing) showed a mean T (m) of 77.0 degrees C. The detected mean difference of the T (m) of 0.6 degrees C between G1 and G2/G3 genotypes might allow a fast and simple discrimination of these genotypes. In conclusion, the real-time PCR developed in the present study provides a powerful tool for molecular studies on E. granulosus with possibilities for extension to other genotypes using different molecular targets.

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Year:  2009        PMID： 19288134     DOI： 10.1007/s00436-009-1388-y

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  18 in total

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Journal:  Microbiol Immunol       Date:  2005       Impact factor: 1.955

Review 2.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

3.  Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis.

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Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

4.  Oligonucleotide melting temperatures under PCR conditions: nearest-neighbor corrections for Mg(2+), deoxynucleotide triphosphate, and dimethyl sulfoxide concentrations with comparison to alternative empirical formulas.

Authors:  N von Ahsen; C T Wittwer; E Schütz
Journal:  Clin Chem       Date:  2001-11       Impact factor: 8.327

5.  Evaluation of three PCR assays for the identification of the sheep strain (genotype 1) of Echinococcus granulosus in canid feces and parasite tissues.

Authors:  Belgees S Boufana; Maiza Campos-Ponce; Ariel Naidich; Imad Buishi; Selma Lahmar; Eberhard Zeyhle; David J Jenkins; Benoit Combes; Hao Wen; Ning Xiao; Minoru Nakao; Akira Ito; Jiamin Qiu; Philip S Craig
Journal:  Am J Trop Med Hyg       Date:  2008-05       Impact factor: 2.345

6.  Cystic echinococcosis in water buffaloes: epidemiological survey and molecular evidence of ovine (G1) and buffalo (G3) strains.

Authors:  F Capuano; L Rinaldi; M P Maurelli; A G Perugini; V Veneziano; G Garippa; C Genchi; V Musella; G Cringoli
Journal:  Vet Parasitol       Date:  2006-02-09       Impact factor: 2.738

7.  Molecular update on cystic echinococcosis in cattle and water buffaloes of southern Italy.

Authors:  L Rinaldi; M P Maurelli; F Capuano; A G Perugini; V Veneziano; S Cringoli
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8.  A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa.

Authors:  Anke Dinkel; Ernest M Njoroge; Anja Zimmermann; Marcus Wälz; Eberhard Zeyhle; Ibrahim E Elmahdi; Ute Mackenstedt; Thomas Romig
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Review 9.  Molecular epidemiology of cystic echinococcosis.

Authors:  D P McManus; R C A Thompson
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10.  A quantitative real-time PCR assay for the detection of tetR of Tn10 in Escherichia coli using SYBR Green and the Opticon.

Authors:  Christian Morsczeck; Daniel Langendörfer; Jörg Michael Schierholz
Journal:  J Biochem Biophys Methods       Date:  2004-06-30
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  7 in total

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Journal:  Parasitol Res       Date:  2013-07-07       Impact factor: 2.289

2.  Is the goat a new host for the G3 Indian buffalo strain of Echinococcus granulosus?

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Journal:  ScientificWorldJournal       Date:  2012-05-15

3.  Increase of Vascular Endothelial Growth Factor and Decrease of MCP-1 and Some Updated Epidemiology Aspects of Cystic Echinococcosis Human Cases in Calabria Region.

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Journal:  Mediators Inflamm       Date:  2018-01-14       Impact factor: 4.711

4.  First report of Tasmanian sheep strain (G2) genotype isolated from Iranian goat using the high resolution melting (HRM) analysis.

Authors:  Ahmad Hosseini-Safa; Mohammad Ali Mohag Hegh; Nader Pestechian; Maryam Ganji; Rasoul Mohammadi; Reza Mahmoudi Lamouki; Mohammad Rostami-Nejad
Journal:  Gastroenterol Hepatol Bed Bench       Date:  2016-12

5.  Echinococcus granulosus sensu lato Genotypes in Different Hosts Worldwide: A Systematic Review.

Authors:  Carlos Manterola; Armando Totomoch-Serra; Claudio Rojas; Ángela L Riffo-Campos; Nayely García-Méndez
Journal:  Acta Parasitol       Date:  2021-07-15       Impact factor: 1.440

6.  Genetic diversity of Echinococcus granulosus in center of Iran.

Authors:  Nader Pestechian; Ahmad Hosseini Safa; Mohammadhasan Tajedini; Mohammad Rostami-Nejad; Mohammad Mousavi; Hosseinali Yousofi; Shaghayegh Haghjooy Javanmard
Journal:  Korean J Parasitol       Date:  2014-08-29       Impact factor: 1.341

7.  Development and evaluation of real-time loop-mediated isothermal amplification assay for rapid detection of cystic echinococcosis.

Authors:  Mohamed E Ahmed; Mawahib H Eldigail; Fatima M Elamin; Ibtisam A Ali; Martin P Grobusch; Imadeldin E Aradaib
Journal:  BMC Vet Res       Date:  2016-09-13       Impact factor: 2.741
  7 in total

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