Literature DB >> 19287626

Nematode autofluorescence and its use as an indicator of viability.

T A Forge, A E Macguidwin.   

Abstract

Representatives of 15 nematode genera were viewed with 450-490-nm epi-illumination and found to autofluoresce. The autofluorescence was limited to 1-5-mum-d globules in the intestinal cells of live nematodes. When adult Pratylenchus penetrans or Caenorhabditis elegans were killed with formaldehyde, freezing, or heat, autofluorescence dispersed throughout the body. Mixed stages of P. penetrans were killed by freezing at several different temperatures. Estimates of survival based on autofluorescence dispersal matched estimates based on mobility more closely than did estimates based on the vital stain, eosin-y.

Entities:  

Keywords:  autofluorescence; lipofuscin; nematode viability; secondary lysosome

Year:  1989        PMID： 19287626      PMCID： PMC2618949     

Source DB:  PubMed          Journal:  J Nematol        ISSN： 0022-300X            Impact factor:   1.402


  10 in total

1.  Propidium iodide enabled live imaging of Pasteuria sp.-Pratylenchus zeae infection studies under fluorescence microscopy.

Authors:  Francine Perrine-Walker; Khoa Le
Journal:  Protoplasma       Date:  2020-10-17       Impact factor: 3.356

2.  Improvement of crystal solubility and increasing toxicity against Caenorhabditis elegans by asparagine substitution in block 3 of Bacillus thuringiensis crystal protein Cry5Ba.

Authors:  Fenshan Wang; Yingying Liu; Fengjuan Zhang; Lujun Chai; Lifang Ruan; Donghai Peng; Ming Sun
Journal:  Appl Environ Microbiol       Date:  2012-08-03       Impact factor: 4.792

3.  Distinct temporal actions of different types of unfolded protein responses during aging.

Authors:  Yi Sheng; Guang Yang; Zachary Markovich; Sung Min Han; Rui Xiao
Journal:  J Cell Physiol       Date:  2020-12-21       Impact factor: 6.513

4.  New live screening of plant-nematode interactions in the rhizosphere.

Authors:  Felicity E O'Callaghan; Roberto A Braga; Roy Neilson; Stuart A MacFarlane; Lionel X Dupuy
Journal:  Sci Rep       Date:  2018-01-23       Impact factor: 4.379

5.  Reduction of autofluorescence in whole adult worms of Schistosoma japonicum for immunofluorescence assay.

Authors:  Qunfeng Wu; Zheng Feng; Wei Hu
Journal:  Parasit Vectors       Date:  2021-10-14       Impact factor: 3.876

6.  In vivo imaging of fluorescent single-walled carbon nanotubes within C. elegans nematodes in the near-infrared window.

Authors:  Adi Hendler-Neumark; Verena Wulf; Gili Bisker
Journal:  Mater Today Bio       Date:  2021-12-02

7.  Fluoroalkenyl-Grafted Chitosan Oligosaccharide Derivative: An Exploration for Control Nematode Meloidogyne Incognita.

Authors:  Zhaoqian Fan; Yukun Qin; Song Liu; Ronge Xing; Huahua Yu; Kecheng Li; Pengcheng Li
Journal:  Int J Mol Sci       Date:  2022-02-14       Impact factor: 5.923

8.  Autofluorescence as a measure of senescence in C. elegans: look to red, not blue or green.

Authors:  Zachary Pincus; Travis C Mazer; Frank J Slack
Journal:  Aging (Albany NY)       Date:  2016-05       Impact factor: 5.682

9.  Using C. elegans Forward and Reverse Genetics to Identify New Compounds with Anthelmintic Activity.

Authors:  Mark D Mathew; Neal D Mathew; Angela Miller; Mike Simpson; Vinci Au; Stephanie Garland; Marie Gestin; Mark L Edgley; Stephane Flibotte; Aruna Balgi; Jennifer Chiang; Guri Giaever; Pamela Dean; Audrey Tung; Michel Roberge; Calvin Roskelley; Tom Forge; Corey Nislow; Donald Moerman
Journal:  PLoS Negl Trop Dis       Date:  2016-10-18

10.  Bacopa monnieri (L.) wettst. Extract protects against glutamate toxicity and increases the longevity of Caenorhabditis elegans.

Authors:  James Michael Brimson; Mani Iyer Prasanth; Waluga Plaingam; Tewin Tencomnao
Journal:  J Tradit Complement Med       Date:  2019-10-04
  10 in total

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