| Literature DB >> 19286430 |
Takashi Nagashima1, Takahiko Aoyama, Akiko Fukasawa, Satoshi Watabe, Noboru Fukuda, Takahiro Ueno, Hiroshi Sugiyama, Hiroki Nagase, Yoshiaki Matsumoto.
Abstract
Pyrrole (Py)-imidazole (Im) polyamides synthesized by combining N-methylpyrrole and N-methylimidazole amino acids have been identified as novel candidates for gene therapy. In this study, a sensitive method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with an electrospray ionization (ESI) source was developed and validated for the determination and quantification of Py-Im polyamide in rat plasma. Py-Im polyamide was extracted from rat plasma by solid-phase extraction (SPE) using a Waters Oasis HLB cartridge. Separation was achieved on an ACQUITY UPLC HSS T3 (1.8 microm, 2.1 x 50 mm) column by gradient elution using acetonitrile:distilled water:acetic acid (5:95:0.1, v/v/v) and acetonitrile:distilled water:acetic acid (95:5:0.1, v/v/v). The method was validated over the range of 10-1000 ng/mL and the lower limit of quantification (LLOQ) was 10 ng/mL. This method was successfully applied to the investigation of the pharmacokinetics of Py-Im polyamide after intravenous administration.Entities:
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Year: 2009 PMID： 19286430 DOI： 10.1016/j.jchromb.2009.02.041
Source DB: PubMed Journal: J Chromatogr B Analyt Technol Biomed Life Sci ISSN： 1570-0232 Impact factor: 3.205