| Literature DB >> 19285445 |
Kazimierz T Tycowski1, Mei-Di Shu, Abiodun Kukoyi, Joan A Steitz.
Abstract
Small Cajal body (CB)-specific RNPs (scaRNPs) function in posttranscriptional modification of small nuclear (sn)RNAs. An RNA element, the CAB box, facilitates CB localization of H/ACA scaRNPs. Using a related element in Drosophila C/D scaRNAs, we purified a fly WD40 repeat protein that UV crosslinks to RNA in a C/D CAB box-dependent manner and associates with C/D and mixed domain C/D-H/ACA scaRNAs. Its human homolog, WDR79, associates with C/D, H/ACA, and mixed domain scaRNAs, as well as with telomerase RNA. WDR79's binding to human H/ACA and mixed domain scaRNAs is CAB box dependent, and its association with mixed domain RNAs also requires the ACA motif, arguing for additional interactions of WDR79 with H/ACA core proteins. We demonstrate a requirement for WDR79 binding in the CB localization of a scaRNA. This and other recent reports establish WDR79 as a central player in the localization and processing of nuclear RNPs.Entities:
Mesh：
Substances：
Year: 2009 PMID： 19285445 PMCID： PMC2700737 DOI： 10.1016/j.molcel.2009.02.020
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970