Literature DB >> 19282254

A modified extraction protocol enables detection and quantification of celiac disease-related gluten proteins from wheat.

Hetty C van den Broeck1, Antoine H P America, Marinus J M Smulders, Dirk Bosch, Rob J Hamer, Ludovicus J W J Gilissen, Ingrid M van der Meer.   

Abstract

The detection, analysis, and quantification of individual celiac disease (CD) immune responsive gluten proteins in wheat and related cereals (barley, rye) require an adequate and reliable extraction protocol. Because different types of gluten proteins behave differently in terms of solubility, currently different extraction protocols exist. The performance of various documented gluten extraction protocols is evaluated for specificity and completeness by gel electrophoresis (SDS-PAGE), immunoblotting and RIDASCREEN Gliadin competitive ELISA. Based on these results, an optimized, two-step extraction protocol has been developed.

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Year:  2009        PMID： 19282254     DOI： 10.1016/j.jchromb.2009.02.035

Source DB:  PubMed          Journal:  J Chromatogr B Analyt Technol Biomed Life Sci        ISSN： 1570-0232            Impact factor:   3.205


  12 in total

1.  Development of hypoimmunogenic muffins: batter rheology, quality characteristics, microstructure and immunochemical validation.

Authors:  K Umashankar; Jyotsna Rajiv; P Prabhasankar
Journal:  J Food Sci Technol       Date:  2015-09-15       Impact factor: 2.701

2.  Effect of extrusion on the modification of wheat flour proteins related to celiac disease.

Authors:  Yong Wu; Zhiwen Xiao; Xu Jiang; Chongfu Lv; Jinyan Gao; Juanli Yuan; Lingke Shan; Hongbing Chen
Journal:  J Food Sci Technol       Date:  2021-10-11       Impact factor: 3.117

3.  Cloning, Expression, Sequence Analysis and Homology Modeling of the Prolyl Endoprotease from Eurygaster integriceps Puton.

Authors:  Ravi Chandra Yandamuri; Ranjeeta Gautam; Charles Darkoh; Vanitha Dareddy; Mustapha El-Bouhssini; Beatrice A Clack
Journal:  Insects       Date:  2014-10-22       Impact factor: 2.769

4.  Using 7 cm immobilized pH gradient strips to determine levels of clinically relevant proteins in wheat grain extracts.

Authors:  Sona Fekecsová; Maksym Danchenko; Lubica Uvackova; Ludovit Skultety; Martin Hajduch
Journal:  Front Plant Sci       Date:  2015-06-12       Impact factor: 5.753

5.  Rapid and Easy High-Molecular-Weight Glutenin Subunit Identification System by Lab-on-a-Chip in Wheat (Triticum aestivum L.).

Authors:  Dongjin Shin; Jin-Kyung Cha; So-Myeong Lee; Nkulu Rolly Kabange; Jong-Hee Lee
Journal:  Plants (Basel)       Date:  2020-11-09

6.  Omega-5 and Gamma Gliadin are the Major Allergens in Adult-Onset IgE-Mediated Wheat Allergy: Results from Thai Cohort with Oral Food Challenge.

Authors:  Surapon Piboonpocanun; Torpong Thongngarm; Chamard Wongsa; Punchama Pacharn; Onrapak Reamtong; Mongkhon Sompornrattanaphan
Journal:  J Asthma Allergy       Date:  2021-07-15

7.  A Case Study of the Response of Immunogenic Gluten Peptides to Sourdough Proteolysis.

Authors:  Olivia J Ogilvie; Juliet A Gerrard; Sarah Roberts; Kevin H Sutton; Nigel Larsen; Laura J Domigan
Journal:  Nutrients       Date:  2021-06-01       Impact factor: 5.717

8.  Removing celiac disease-related gluten proteins from bread wheat while retaining technological properties: a study with Chinese Spring deletion lines.

Authors:  Hetty C van den Broeck; Teun W J M van Herpen; Cees Schuit; Elma M J Salentijn; Liesbeth Dekking; Dirk Bosch; Rob J Hamer; Marinus J M Smulders; Ludovicus J W J Gilissen; Ingrid M van der Meer
Journal:  BMC Plant Biol       Date:  2009-04-07       Impact factor: 4.215

9.  Analysis of wheat prolamins, the causative agents of celiac sprue, using reversed phase high performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS).

Authors:  Jaime H Mejías; Xiaoqiao Lu; Claudia Osorio; Jeffrey L Ullman; Diter von Wettstein; Sachin Rustgi
Journal:  Nutrients       Date:  2014-04-15       Impact factor: 5.717

10.  Prolamin Content and Grain Weight in RNAi Silenced Wheat Lines Under Different Conditions of Temperature and Nitrogen Availability.

Authors:  Miriam Marín-Sanz; María J Giménez; Francisco Barro; Roxana Savin
Journal:  Front Plant Sci       Date:  2020-03-20       Impact factor: 5.753
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