Literature DB >> 19280259

Carbon-13 (13C) labeling of Bacillus subtilis vegetative cells and spores: suitability for DNA stable isotope probing (DNA-SIP) of spores in soils.

Wayne L Nicholson1, Jeffrey Fedenko, Andrew C Schuerger.   

Abstract

To test the suitability of DNA stable isotope probing (DNA-SIP) for characterizing bacterial spore populations in soils, the properties of Bacillus subtilis cells and spores intensely labeled with [(13)C]glucose were characterized. Spore germination, vegetative growth rates, and sporulation efficiency were indistinguishable on glucose versus [(13)C]glucose, as were spore wet heat and UV resistance. Unlabeled and (13)C-labeled spores contained 1.0989 and 74.336 at.% (13)C, and exhibited wet densities of 1.356 and 1.365 g/ml, respectively. Chromosomal DNAs containing (12)C versus (13)C were readily separated by their different buoyant densities in cesium chloride/ethidium bromide gradients.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19280259     DOI： 10.1007/s00284-009-9387-x

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  19 in total

1.  Method for purification of bacterial endospores from soils: UV resistance of natural Sonoran desert soil populations of Bacillus spp. with reference to B. subtilis strain 168.

Authors:  W L Nicholson; J F Law
Journal:  J Microbiol Methods       Date:  1999-02       Impact factor: 2.363

2.  Using thermal inactivation kinetics to calculate the probability of extreme spore longevity: implications for paleomicrobiology and lithopanspermia.

Authors:  Wayne L Nicholson
Journal:  Orig Life Evol Biosph       Date:  2003-12       Impact factor: 1.950

3.  Microbial characterization of the Mars Odyssey spacecraft and its encapsulation facility.

Authors:  Myron T La Duc; Wayne Nicholson; Roger Kern; Kasthuri Venkateswaran
Journal:  Environ Microbiol       Date:  2003-10       Impact factor: 5.491

4.  Catabolic repression of bacterial sporulation.

Authors:  P Schaeffer; J Millet; J P Aubert
Journal:  Proc Natl Acad Sci U S A       Date:  1965-09       Impact factor: 11.205

5.  Molecular microbial diversity of a spacecraft assembly facility.

Authors:  K Venkateswaran; M Satomi; S Chung; R Kern; R Koukol; C Basic; D White
Journal:  Syst Appl Microbiol       Date:  2001-07       Impact factor: 4.022

6.  Recurrent isolation of hydrogen peroxide-resistant spores of Bacillus pumilus from a spacecraft assembly facility.

Authors:  Michael J Kempf; Fei Chen; Roger Kern; Kasthuri Venkateswaran
Journal:  Astrobiology       Date:  2005-06       Impact factor: 4.335

7.  A soluble protein is immobile in dormant spores of Bacillus subtilis but is mobile in germinated spores: implications for spore dormancy.

Authors:  Ann E Cowan; Dennis E Koppel; Barbara Setlow; Peter Setlow
Journal:  Proc Natl Acad Sci U S A       Date:  2003-03-19       Impact factor: 11.205

8.  Analysis of methanotrophic bacteria in Movile Cave by stable isotope probing.

Authors:  Elena Hutchens; Stefan Radajewski; Marc G Dumont; Ian R McDonald; J Colin Murrell
Journal:  Environ Microbiol       Date:  2004-02       Impact factor: 5.491

9.  Small-Scale DNA Sample Preparation Method for Field PCR Detection of Microbial Cells and Spores in Soil.

Authors: 
Journal:  Appl Environ Microbiol       Date:  1998-07-01       Impact factor: 4.792

10.  Spore UV and acceleration resistance of endolithic Bacillus pumilus and Bacillus subtilis isolates obtained from Sonoran desert basalt: implications for lithopanspermia.

Authors:  James N Benardini; John Sawyer; Kasthuri Venkateswaran; Wayne L Nicholson
Journal:  Astrobiology       Date:  2003       Impact factor: 4.335
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.