| Literature DB >> 19278707 |
C Marchioni1, E Riccardi, S Spinelli, F Dell'Unto, P Grimaldi, A Bedini, C Giliberti, L Giuliani, R Palomba, A Congiu Castellano.
Abstract
The structural effect induced by therapeutic ultrasound on proteins in aqueous solution has been investigated with FTIR spectroscopy, UV-VIS spectroscopy, circular dichroism and light scattering. Six proteins (cytochrome, lysozyme, myoglobin, bovine serum albumin, trypsinogen, and alpha-chymotrypsinogen A) with different molecular weight and secondary structure have been studied. The experiment has been performed using an ultrasound source at resonant frequency of 1 MHz and sonication times of 10, 20, 30, 40, 50, and 60 min. A different behaviour of proteins under sonication depends on the dominant secondary structure type (alpha-helix or beta-sheets) and on the grade of the ordered structure. The results suggest that the free radicals, produced by water sonolysis, have an important role in the changes of structural order.Entities:
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Year: 2009 PMID： 19278707 DOI： 10.1016/j.ultras.2009.02.003
Source DB: PubMed Journal: Ultrasonics ISSN： 0041-624X Impact factor: 2.890