Effects of 20-hydroxyecdysone and juvenile hormone on octopamine metabolism in females of Drosophila.

The effect of exogenous 20-hydroxyecdysone (20E) and juvenile hormone (JH) on the activities of the tyrosine decarboxylase (TDC), the first enzyme in octopamine (OA) synthesis, has been studied in young females of wild type D. virilis and D. melanogaster under normal and heat stress (38 degrees C) conditions. Flies fed 20E expressed increased TDC activity in both species. JH application decreased TDC activity in both species. A rise in JH and 20E levels did not prevent a TDC response to heat stress, but changed the response intensity. A long-term increase in JH titre had no effect on the activity of main OA catabolyzing enzyme, arylalkylamine N-acetyltransferase, in females of both species. A possible mechanism of regulation of OA levels by 20E and JH in Drosophila females is discussed.