AIMS: With three consecutive tetratricopeptide repeat (TPR) motifs at its C-terminus essential for neuronal migration, and a p23 domain at its N-terminus, DYX1C1 was the first gene proposed to have a role in developmental dyslexia. In this study, we attempted to identify the potential interaction of DYX1C1 and heat shock protein, and the role of DYX1C1 in breast cancer. MAIN METHODS: GST pull-down, a yeast two-hybrid system, RT-PCR, site-directed mutagenesis approach. KEY FINDINGS: Our study initially confirmed DYX1C1, a dyslexia related protein, could interact with Hsp70 and Hsp90 via GST pull-down and a yeast two-hybrid system. And we verified that EEVD, the C-terminal residues of DYX1C1, is responsible for the identified association. Further, DYX1C1 mRNA was significantly overexpressed in malignant breast tumor, linking with the up-regulated expression of Hsp70 and Hsp90. SIGNIFICANCE: These results suggest that DYX1C1 is a novel Hsp70 and Hsp90-interacting co-chaperone protein and its expression is associated with malignancy.
AIMS: With three consecutive tetratricopeptide repeat (TPR) motifs at its C-terminus essential for neuronal migration, and a p23 domain at its N-terminus, DYX1C1 was the first gene proposed to have a role in developmental dyslexia. In this study, we attempted to identify the potential interaction of DYX1C1 and heat shock protein, and the role of DYX1C1 in breast cancer. MAIN METHODS: GST pull-down, a yeast two-hybrid system, RT-PCR, site-directed mutagenesis approach. KEY FINDINGS: Our study initially confirmed DYX1C1, a dyslexia related protein, could interact with Hsp70 and Hsp90 via GST pull-down and a yeast two-hybrid system. And we verified that EEVD, the C-terminal residues of DYX1C1, is responsible for the identified association. Further, DYX1C1 mRNA was significantly overexpressed in malignant breast tumor, linking with the up-regulated expression of Hsp70 and Hsp90. SIGNIFICANCE: These results suggest that DYX1C1 is a novel Hsp70 and Hsp90-interacting co-chaperone protein and its expression is associated with malignancy.
Authors: Y Wang; M Paramasivam; A Thomas; J Bai; N Kaminen-Ahola; J Kere; J Voskuil; G D Rosen; A M Galaburda; J J Loturco Journal: Neuroscience Date: 2006-09-20 Impact factor: 3.590
Authors: Kristiina Tammimies; Isabel Tapia-Páez; Joëlle Rüegg; Gustaf Rosin; Juha Kere; Jan-Åke Gustafsson; Ivan Nalvarte Journal: Mol Endocrinol Date: 2012-03-01
Authors: Margaret W Leigh; Amjad Horani; BreAnna Kinghorn; Michael G O'Connor; Maimoona A Zariwala; Michael R Knowles Journal: Transl Sci Rare Dis Date: 2019-07-04
Authors: Amjad Horani; Alessandro Ustione; Tao Huang; Amy L Firth; Jiehong Pan; Sean P Gunsten; Jeffrey A Haspel; David W Piston; Steven L Brody Journal: Proc Natl Acad Sci U S A Date: 2018-01-22 Impact factor: 11.205
Authors: Aarti Tarkar; Niki T Loges; Christopher E Slagle; Richard Francis; Gerard W Dougherty; Joel V Tamayo; Brett Shook; Marie Cantino; Daniel Schwartz; Charlotte Jahnke; Heike Olbrich; Claudius Werner; Johanna Raidt; Petra Pennekamp; Marouan Abouhamed; Rim Hjeij; Gabriele Köhler; Matthias Griese; You Li; Kristi Lemke; Nikolas Klena; Xiaoqin Liu; George Gabriel; Kimimasa Tobita; Martine Jaspers; Lucy C Morgan; Adam J Shapiro; Stef J F Letteboer; Dorus A Mans; Johnny L Carson; Margaret W Leigh; Whitney E Wolf; Serafine Chen; Jane S Lucas; Alexandros Onoufriadis; Vincent Plagnol; Miriam Schmidts; Karsten Boldt; Ronald Roepman; Maimoona A Zariwala; Cecilia W Lo; Hannah M Mitchison; Michael R Knowles; Rebecca D Burdine; Joseph J Loturco; Heymut Omran Journal: Nat Genet Date: 2013-07-21 Impact factor: 38.330
Authors: Gustaf Rosin; Ulf Hannelius; Linda Lindström; Per Hall; Jonas Bergh; Johan Hartman; Juha Kere Journal: BMC Cancer Date: 2012-02-29 Impact factor: 4.430
Authors: Chiara Olcese; Mitali P Patel; Amelia Shoemark; Santeri Kiviluoto; Marie Legendre; Hywel J Williams; Cara K Vaughan; Jane Hayward; Alice Goldenberg; Richard D Emes; Mustafa M Munye; Laura Dyer; Thomas Cahill; Jeremy Bevillard; Corinne Gehrig; Michel Guipponi; Sandra Chantot; Philippe Duquesnoy; Lucie Thomas; Ludovic Jeanson; Bruno Copin; Aline Tamalet; Christel Thauvin-Robinet; Jean-François Papon; Antoine Garin; Isabelle Pin; Gabriella Vera; Paul Aurora; Mahmoud R Fassad; Lucy Jenkins; Christopher Boustred; Thomas Cullup; Mellisa Dixon; Alexandros Onoufriadis; Andrew Bush; Eddie M K Chung; Stylianos E Antonarakis; Michael R Loebinger; Robert Wilson; Miguel Armengot; Estelle Escudier; Claire Hogg; Serge Amselem; Zhaoxia Sun; Lucia Bartoloni; Jean-Louis Blouin; Hannah M Mitchison Journal: Nat Commun Date: 2017-02-08 Impact factor: 14.919