Literature DB >> 19277563

Construction of cDNA libraries: focus on protists and fungi.

Naiara Rodríguez-Ezpeleta1, Shona Teijeiro, Lise Forget, Gertraud Burger, B Franz Lang.   

Abstract

Sequencing of cDNA libraries is an efficient and inexpensive approach to analyze the protein-coding portion of a genome. It is frequently used for surveying the genomes of poorly studied eukaryotes, and is particularly useful for species that are not easily amenable to genome sequencing, because they are nonaxenic and/or difficult to cultivate. In this chapter, we describe protocols that have been applied successfully to construct and normalize a variety of cDNA libraries from many different species of free-living protists and fungi, and that require only small quantities of cell material.

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Year:  2009        PMID： 19277563     DOI： 10.1007/978-1-60327-136-3_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  RNA-level unscrambling of fragmented genes in Diplonema mitochondria.

Authors:  Georgette N Kiethega; Yifei Yan; Marcel Turcotte; Gertraud Burger
Journal:  RNA Biol       Date:  2013-01-16       Impact factor: 4.652

Review 2.  Whole transcriptome analysis with sequencing: methods, challenges and potential solutions.

Authors:  Zhihua Jiang; Xiang Zhou; Rui Li; Jennifer J Michal; Shuwen Zhang; Michael V Dodson; Zhiwu Zhang; Richard M Harland
Journal:  Cell Mol Life Sci       Date:  2015-05-28       Impact factor: 9.261

3.  Construction and identification of a cDNA library for use in the yeast two-hybrid system from duck embryonic fibroblast cells post-infected with duck enteritis virus.

Authors:  Xinghong Gao; Renyong Jia; Mingshu Wang; Dekang Zhu; Shun Chen; Meng Lin; Zhongqiong Yin; Yin Wang; Xiaoyue Chen; Anchun Cheng
Journal:  Mol Biol Rep       Date:  2013-11-30       Impact factor: 2.316

4.  An Unexpectedly Complex Mitoribosome in Andalucia godoyi, a Protist with the Most Bacteria-like Mitochondrial Genome.

Authors:  Matus Valach; José Angel Gonzalez Alcazar; Matt Sarrasin; B Franz Lang; Michael W Gray; Gertraud Burger
Journal:  Mol Biol Evol       Date:  2021-03-09       Impact factor: 16.240

5.  Alternative SARS-CoV-2 detection protocol from self-collected saliva for mass diagnosis and epidemiological studies in low-incoming regions.

Authors:  Luana Prado Rolim de Oliveira; Aline Diniz Cabral; Andreia Moreira Dos Santos Carmo; Adriana Feliciano Duran; Diego Marin Fermino; Glaucia Raquel Luciano Veiga; Beatriz da Costa Aguiar Alves; Carla Moreira Santana; Felipe Baena Garcia; Edmar Silva Santos; Felipe Trovalim Jordão; Andressa Moreira Siqueira; Ivana Barros de Campos; Daniela Rodrigues Colpas; Fernanda Nascimento Almeida; Fernando Luiz Affonso Fonseca; Márcia Aparecida Sperança
Journal:  J Virol Methods       Date:  2021-11-27       Impact factor: 2.014

Review 6.  Characterization of Two Novel Toti-Like Viruses Co-infecting the Atlantic Blue Crab, Callinectes sapidus, in Its Northern Range of the United States.

Authors:  Mingli Zhao; Lan Xu; Holly Bowers; Eric J Schott
Journal:  Front Microbiol       Date:  2022-03-03       Impact factor: 5.640

7.  Phylogenomic analyses support the monophyly of Taphrinomycotina, including Schizosaccharomyces fission yeasts.

Authors:  Yu Liu; Jessica W Leigh; Henner Brinkmann; Melanie T Cushion; Naiara Rodriguez-Ezpeleta; Hervé Philippe; B Franz Lang
Journal:  Mol Biol Evol       Date:  2008-10-14       Impact factor: 16.240

8.  Novel modes of RNA editing in mitochondria.

Authors:  Sandrine Moreira; Matus Valach; Mohamed Aoulad-Aissa; Christian Otto; Gertraud Burger
Journal:  Nucleic Acids Res       Date:  2016-03-21       Impact factor: 16.971

9.  Keeping it complicated: Mitochondrial genome plasticity across diplonemids.

Authors:  Matus Valach; Sandrine Moreira; Steve Hoffmann; Peter F Stadler; Gertraud Burger
Journal:  Sci Rep       Date:  2017-10-26       Impact factor: 4.379
  9 in total

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