PURPOSE: The antiviral activity of an established antibacterial CAP37 domain and its extracellular mechanism of action were investigated. METHODS: CAP37-derived peptides modified to assess the importance of disulfide bonds were evaluated in cytotoxicity and antiviral assays (direct time kill, dose dependency, and TOTO-1) for adenovirus (Ad) and herpes simplex virus type 1 (HSV-1). RESULTS: Variable virus, adenovirus serotype-dependent, and dose-dependent inhibition were demonstrated without cytotoxicity. For peptide A (CAP37(20-44)), TOTO-1 dye uptake was demonstrated for Ad5 and HSV-1. CONCLUSIONS: Unlike the antibacterial activity of this CAP37 domain, its antiviral activity is not fully dependent upon disulfide bond formation. Viral inhibition appears to result, in part, from disruption of the envelope and/or capsid.
PURPOSE: The antiviral activity of an established antibacterial CAP37 domain and its extracellular mechanism of action were investigated. METHODS:CAP37-derived peptides modified to assess the importance of disulfide bonds were evaluated in cytotoxicity and antiviral assays (direct time kill, dose dependency, and TOTO-1) for adenovirus (Ad) and herpes simplex virus type 1 (HSV-1). RESULTS: Variable virus, adenovirus serotype-dependent, and dose-dependent inhibition were demonstrated without cytotoxicity. For peptide A (CAP37(20-44)), TOTO-1 dye uptake was demonstrated for Ad5 and HSV-1. CONCLUSIONS: Unlike the antibacterial activity of this CAP37 domain, its antiviral activity is not fully dependent upon disulfide bond formation. Viral inhibition appears to result, in part, from disruption of the envelope and/or capsid.
Authors: Stephen A K Harvey; Eric G Romanowski; Kathleen A Yates; Y Jerold Gordon Journal: Invest Ophthalmol Vis Sci Date: 2005-10 Impact factor: 4.799
Authors: Zhenping Wang; Yuping Lai; Jamie J Bernard; Daniel T Macleod; Anna L Cogen; Bernard Moss; Anna Di Nardo Journal: J Immunol Date: 2011-12-02 Impact factor: 5.422
Authors: Shaun K Morris; Lisa G Pell; Mohammed Ziaur Rahman; Michelle C Dimitris; Abdullah Mahmud; M Munirul Islam; Tahmeed Ahmed; Eleanor Pullenayegum; Tahmid Kashem; Shaila S Shanta; Jonathan Gubbay; Eszter Papp; Michelle Science; Stanley Zlotkin; Daniel E Roth Journal: BMC Pregnancy Childbirth Date: 2016-10-13 Impact factor: 3.007
Authors: Michelle Science; Jonathon L Maguire; Margaret L Russell; Marek Smieja; Stephen D Walter; Mark Loeb Journal: Clin Infect Dis Date: 2013-05-15 Impact factor: 9.079
Authors: Kelly C L Mulder; Loiane A Lima; Vivian J Miranda; Simoni C Dias; Octávio L Franco Journal: Front Microbiol Date: 2013-10-31 Impact factor: 5.640