| Literature DB >> 19272351 |
Clara Musicco1, Valentina Capelli, Vito Pesce, Anna Maria Timperio, Menotti Calvani, Luigi Mosconi, Lello Zolla, Palmiro Cantatore, Maria Nicola Gadaleta.
Abstract
Overoxidation and subsequent inactivation of Peroxiredoxin III (PrxIII), a mitochondrial H(2)O(2) scavenging enzyme, have been reported in oxidative stress conditions. No data are available in the literature about the presence of overoxidized forms of PrxIII in aged tissues. Liver mitochondria from 12-month-old rats and 28-month-old rats were here analyzed by two-dimensional gel electrophoresis. A spot corresponding to the native form of PrxIII was present in adult and old rats with the same volume, whereas an additional, more acidic spot, of the same molecular weight of the native form, accumulated only in old rats. The acidic spot was identified, by MALDI-MS analysis, as a form of PrxIII bearing the cysteine of the catalytic site overoxidized to sulphonic acid. This modified PrxIII form corresponds to the irreversibly inactivated enzyme, here reported, for the first time, in aging. Three groups of 28-month-old rats treated with acetyl-l-carnitine were also examined. Reduced accumulation of the overoxidized PrxIII form was found in all ALCAR-treated groups.Entities:
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Year: 2009 PMID： 19272351 DOI： 10.1016/j.bbabio.2009.03.002
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002