BACKGROUND: Drug resistance monitoring is an important aspect of tuberculosis (TB) control. OBJECTIVE: To determine the sensitivity of mycobacterial isolates from newly diagnosed pulmonary TB patients to first line anti-TB drugs in Addis Ababa. METHODS: A survey on primary anti-tuberculosis drug resistance was conducted on smear positive pulmonary TB patients visiting 19 health centers and 3 hospitals in Addis Ababa from September 2004 to December 2005. Sputum was digested and decontaminated using Petroff's method and inoculated on Lowenstein-Jensen (LJ) media. Mycobacterial isolates were tested for sensitivity to isoniazid, rifampicin, ethambutol and streptomycin on Middlebrook 7H10 media using the standard indirect proportion method. RESULTS: Among the M. tuberculosis strains isolated from 173 patients, 21.4% were resistant to at least one drug. Single drug resistance to streptomycin was observed in 16.2%, to isoniazid in 13.3%, to rifampicin in 1.2% and to ethambutol in 3.5% of the isolates. The prevalence of resistance to at least one drug was 15.7% and 23.7% among patients with and without HIV co-infection, respectively (p > 0.05). Only one patient (0.6%) had a multidrug resistant (MDR) strain. However, the prevalence of resistance to more than one drug was 10.4%. CONCLUSION: Although the prevalence of MDR was low in this study, the precursors of MDR are accumulating in Addis Ababa.
BACKGROUND: Drug resistance monitoring is an important aspect of tuberculosis (TB) control. OBJECTIVE: To determine the sensitivity of mycobacterial isolates from newly diagnosed pulmonary TBpatients to first line anti-TB drugs in Addis Ababa. METHODS: A survey on primary anti-tuberculosis drug resistance was conducted on smear positive pulmonary TBpatients visiting 19 health centers and 3 hospitals in Addis Ababa from September 2004 to December 2005. Sputum was digested and decontaminated using Petroff's method and inoculated on Lowenstein-Jensen (LJ) media. Mycobacterial isolates were tested for sensitivity to isoniazid, rifampicin, ethambutol and streptomycin on Middlebrook 7H10 media using the standard indirect proportion method. RESULTS: Among the M. tuberculosis strains isolated from 173 patients, 21.4% were resistant to at least one drug. Single drug resistance to streptomycin was observed in 16.2%, to isoniazid in 13.3%, to rifampicin in 1.2% and to ethambutol in 3.5% of the isolates. The prevalence of resistance to at least one drug was 15.7% and 23.7% among patients with and without HIV co-infection, respectively (p > 0.05). Only one patient (0.6%) had a multidrug resistant (MDR) strain. However, the prevalence of resistance to more than one drug was 10.4%. CONCLUSION: Although the prevalence of MDR was low in this study, the precursors of MDR are accumulating in Addis Ababa.
Authors: Deus Lukoye; Willy Ssengooba; Kenneth Musisi; George W Kasule; Frank G J Cobelens; Moses Joloba; Gabriela B Gomez Journal: BMC Public Health Date: 2015-03-25 Impact factor: 3.295
Authors: Wassihun Wedajo; Thomas Schön; Ahmed Bedru; Teklu Kiros; Elena Hailu; Tesfamariam Mebrahtu; Lawrence Yamuah; Kristian Ängeby; Jim Werngren; Philip Onyebujoh; Kifle Dagne; Abraham Aseffa Journal: BMC Res Notes Date: 2014-08-10