AIM: An in vitro study was carried out to determine the effect of UHRF1 overexpression on radiosensitivity in human cervical cancer HeLa cells using adenovirus-mediated UHRF1 gene transfer (Ad5-UHRF1). METHODS: Cell survival was evaluated using the clonogenic survival assay and the MTT assay; apoptosis and cell cycle distribution were monitored by flow cytometry. Protein levels were measured by Western blotting. Silencing XRCC4 expression was performed by transfection of small interfering RNA (siRNA). RESULTS: Increased expression of UHRF1 by Ad5-UHRF1 significantly reduced the radiosensitivity of HeLa cells. The UHRF1-mediated radioresistance was correlated with increased DNA repair capability and increased expression of the DNA damage repair protein, XRCC4. Knocking down XRCC4 expression in the cells using XRCC4 siRNA markedly reduced the UHRF1-mediated radioresistance. CONCLUSION: These results provide the first evidence for revealing a functional role of UHRF1 in human cervical cancer cells as a negative regulator of radiosensitivity.
AIM: An in vitro study was carried out to determine the effect of UHRF1 overexpression on radiosensitivity in human cervical cancer HeLa cells using adenovirus-mediated UHRF1 gene transfer (Ad5-UHRF1). METHODS: Cell survival was evaluated using the clonogenic survival assay and the MTT assay; apoptosis and cell cycle distribution were monitored by flow cytometry. Protein levels were measured by Western blotting. Silencing XRCC4 expression was performed by transfection of small interfering RNA (siRNA). RESULTS: Increased expression of UHRF1 by Ad5-UHRF1 significantly reduced the radiosensitivity of HeLa cells. The UHRF1-mediated radioresistance was correlated with increased DNA repair capability and increased expression of the DNA damage repair protein, XRCC4. Knocking down XRCC4 expression in the cells using XRCC4 siRNA markedly reduced the UHRF1-mediated radioresistance. CONCLUSION: These results provide the first evidence for revealing a functional role of UHRF1 in human cervical cancer cells as a negative regulator of radiosensitivity.
Authors: Y Gao; D O Ferguson; W Xie; J P Manis; J Sekiguchi; K M Frank; J Chaudhuri; J Horner; R A DePinho; F W Alt Journal: Nature Date: 2000-04-20 Impact factor: 49.962
Authors: K M Frank; J M Sekiguchi; K J Seidl; W Swat; G A Rathbun; H L Cheng; L Davidson; L Kangaloo; F W Alt Journal: Nature Date: 1998-11-12 Impact factor: 49.962
Authors: A Fujimori; Y Matsuda; Y Takemoto; Y Hashimoto; E Kubo; R Araki; R Fukumura; K Mita; K Tatsumi; M Muto Journal: Mamm Genome Date: 1998-12 Impact factor: 2.957
Authors: Ian Marc Bonapace; Lucia Latella; Roberto Papait; Francesco Nicassio; Alessandra Sacco; Masahiro Muto; Marco Crescenzi; Pier Paolo Di Fiore Journal: J Cell Biol Date: 2002-06-10 Impact factor: 10.539
Authors: Mounira Krifa; Mahmoud Alhosin; Christian D Muller; Jean-Pierre Gies; Leila Chekir-Ghedira; Kamel Ghedira; Yves Mély; Christian Bronner; Marc Mousli Journal: J Exp Clin Cancer Res Date: 2013-05-20