| Literature DB >> 192698 |
J M Walboomers, R van Ketel, M J Becker-Bloemkolk.
Abstract
Thymidine kinase (TK) activity of uninfected and in vitro herpes simplex virus type 2 (HSV-2) infected human fetal lung cells was analyzed. Polyacrylamide gel electrophoresis was carried out, and subsequent incubation of equally divided gel slices in the presence of normal or anti-HSV-2 specific TK serum, together with different substrates, was performed. Small amounts of virus-specific enzyme could be detected using this combination of electrophoresis and antibodies and replacing ATP by CTP in the substrate medium.Entities:
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Year: 1977 PMID： 192698 DOI： 10.1159/000148894
Source DB: PubMed Journal: Intervirology ISSN： 0300-5526 Impact factor: 1.763