OBJECTIVE: To investigate the potential role of PArkin co-regulated gene (PACRG) in human male infertility. DESIGN: Case-control study. SETTING: Academic reproductive biology department. PATIENT(S): Blood samples were obtained from 610 patients and 156 normal control subjects. INTERVENTION(S): Genomic DNA was used as template for polymerase chain reaction amplification of the PACRG promoter and coding exons. The amplified fragments were tested for DNA sequence variations by direct sequencing and restriction enzyme analysis. MAIN OUTCOME MEASURE(S): Gene structure and sequence alterations of PACRG in infertile male patients. RESULT(S): The structure of PACRG was determined to comprise 5 coding exons, generating a single transcript in the testis which encoded a predicted protein of 257 amino acids. No pathogenic mutations were identified; however, a variant in the promoter of PACRG was shown to be significantly associated with azoospermia, but not oligospermia, in the case-control cohort. CONCLUSION(S): Mutation of PACRG was not identified as a cause of male infertility, but variation in the promoter was demonstrated to be a risk factor associated with azoospermia. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
OBJECTIVE: To investigate the potential role of PArkin co-regulated gene (PACRG) in humanmale infertility. DESIGN: Case-control study. SETTING: Academic reproductive biology department. PATIENT(S): Blood samples were obtained from 610 patients and 156 normal control subjects. INTERVENTION(S): Genomic DNA was used as template for polymerase chain reaction amplification of the PACRG promoter and coding exons. The amplified fragments were tested for DNA sequence variations by direct sequencing and restriction enzyme analysis. MAIN OUTCOME MEASURE(S): Gene structure and sequence alterations of PACRG in infertile malepatients. RESULT(S): The structure of PACRG was determined to comprise 5 coding exons, generating a single transcript in the testis which encoded a predicted protein of 257 amino acids. No pathogenic mutations were identified; however, a variant in the promoter of PACRG was shown to be significantly associated with azoospermia, but not oligospermia, in the case-control cohort. CONCLUSION(S): Mutation of PACRG was not identified as a cause of male infertility, but variation in the promoter was demonstrated to be a risk factor associated with azoospermia. Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Authors: Jerry Wei; Palaniappan Ramanathan; Peter C Thomson; Ian C Martin; Christopher Moran; Peter Williamson Journal: Mol Biotechnol Date: 2013-02 Impact factor: 2.695
Authors: Zhibing Zhang; Xuening Shen; David R Gude; Bonney M Wilkinson; Monica J Justice; Charles J Flickinger; John C Herr; Edward M Eddy; Jerome F Strauss Journal: Proc Natl Acad Sci U S A Date: 2009-09-17 Impact factor: 11.205
Authors: Wei Li; Waixing Tang; Maria E Teves; Zhengang Zhang; Ling Zhang; Hongfei Li; Kellie J Archer; Darrell L Peterson; David C Williams; Jerome F Strauss; Zhibing Zhang Journal: Development Date: 2015-03-01 Impact factor: 6.868
Authors: Sarah E M Stephenson; Timothy D Aumann; Juliet M Taylor; Jessica R Riseley; Ruili Li; Jeffrey R Mann; Doris Tomas; Paul J Lockhart Journal: Sci Rep Date: 2018-05-14 Impact factor: 4.379