Dose-rate effectiveness for unstable-type chromosome aberrations detected in mice after continuous irradiation with low-dose-rate gamma rays.

Chronological changes in the chromosome aberration rates of splenocytes from specific-pathogen-free (SPF) mice after continuous and long-term exposure to low-dose-rate gamma rays were studied. Incidences of dicentrics plus centric rings (Dic+Rc), detected by conventional Giemsa staining, and dicentric chromosomes, detected by fluorescence in situ hybridization (Dic by FISH) using a centromere probe, showed an essentially linear increase up to a total accumulated dose of 8000 mGy after irradiation for about 400 days at a low dose rate of 20 mGy/day. For comparison, acute high-dose-rate and medium-dose-rate irradiation were performed. The values of the alpha coefficients in the linear regression lines for these unstable-type aberrations decreased as the dose rates were lowered from medium dose rates (200 and 400 mGy/day) to low dose rates (1 and 20 mGy/day). The dose and dose-rate effectiveness factor (DDREF), estimated by the ratio of calculated incidences using the best-fit regression lines at a high dose rate (890 mGy/min) and low dose rate (20 mGy/day), was 4.5 for Dic by FISH and 5.2 for Dic+Rc, respectively, at the same dose of 100 mGy, while different DDREFs were obtained for different accumulated doses. This is the first study to provide information regarding the effects of long-term exposure to low-dose-rate radiation on chromosomes.