Literature DB >> 19265947

Video capture and editing as a tool for the storage, distribution, and illustration of morphological characters of nematodes.

Paul De Ley, Wim Bert.   

Abstract

Morphological identification and detailed observation of nematodes usually requires permanent slides, but these are never truly permanent and often prevent the same specimens to be used for other purposes. To efficiently record the morphology of nematodes in a format that allows easy archiving, editing, and distribution, we have assembled two micrographic video capture and editing (VCE) configurations. These assemblies allow production of short video clips that mimic multifocal observation of nematode specimens through a light microscope. Images so obtained can be used for training, management, and online access of "virtual voucher specimens" in taxonomic collections, routine screening of fixed or unfixed specimens, recording of ephemeral staining patterns, or recording of freshly dissected internal organs prior to their decomposition. We provide an overview of the components and operation of both of our systems and evaluate their efficiency and image quality. We conclude that VCE is a highly versatile approach that is likely to become widely used in nematology research and teaching.

Keywords:  World Wide Web; computer applications; database; image editing; methods; microscopy; morphology; type collections; video

Year:  2002        PMID: 19265947      PMCID: PMC2620590     

Source DB:  PubMed          Journal:  J Nematol        ISSN: 0022-300X            Impact factor:   1.402


  7 in total

1.  Defining operational taxonomic units using DNA barcode data.

Authors:  Mark Blaxter; Jenna Mann; Tom Chapman; Fran Thomas; Claire Whitton; Robin Floyd; Eyualem Abebe
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2005-10-29       Impact factor: 6.237

2.  An integrated approach to fast and informative morphological vouchering of nematodes for applications in molecular barcoding.

Authors:  Paul De Ley; Irma Tandingan De Ley; Krystalynne Morris; Eyualem Abebe; Manuel Mundo-Ocampo; Melissa Yoder; Joseph Heras; Dora Waumann; Axayácatl Rocha-Olivares; A H Jay Burr; James G Baldwin; W Kelley Thomas
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2005-10-29       Impact factor: 6.237

3.  A position paper on the electronic publication of nematode taxonomic manuscripts.

Authors:  James G Baldwin; Byron Adams; Duane Hope; Scott Gardner; Robin Huettel; Peter Mullin; Topper Powers; Jyotsna Sharma; Weimin Ye; William K Thomas
Journal:  J Nematol       Date:  2006-09       Impact factor: 1.402

4.  Utility of Classical α-Taxonomy for Biodiversity of Aquatic Nematodes.

Authors:  Wilfrida Decraemer; Thierry Backeljau
Journal:  J Nematol       Date:  2015-03       Impact factor: 1.402

5.  A technique for making high-resolution megapixel mosaic photomicrographs of nematodes.

Authors:  J D Eisenback
Journal:  J Nematol       Date:  2012-09       Impact factor: 1.402

6.  Redefinition of Genus Malenchus Andrássy, 1968 (Tylenchomorpha: Tylenchidae) with Additional Data on Ecology.

Authors:  Xue Qing; Wim Bert
Journal:  J Nematol       Date:  2017-06       Impact factor: 1.402

7.  The comparative cellular architecture of the female gonoduct among tylenchoidea (nematoda: tylenchina).

Authors:  W Bert; M Claeys; G Borgonie
Journal:  J Nematol       Date:  2006-09       Impact factor: 1.402

  7 in total

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