The early lymphocyte proliferation response in sheep exposed to Mycobacterium avium subsp. paratuberculosis compared to infection status.

Johne's disease (JD) is an enteric mycobacterial infection of ruminants that has significant global economic impact. Not all individuals exposed to pathogenic mycobacteria succumb to disease. Therefore although early detection of infectious individuals is vital, it is equally important to distinguish how the host response differs in those that are able to successfully clear or control mycobacterial infection and those that are unable to do so. To further our understanding of this issue we studied the antigen-specific proliferation of lymphocytes, including lymphocyte subsets, during the course of early experimental ovine paratuberculosis and assessed differences in this response between animals that eventually succumbed to disease and those that remained disease-free. While proliferation of blood lymphocytes was significantly higher in sheep exposed to Mptb compared to unchallenged controls as early as 4 months post inoculation (p.i.), there was no difference in PBMC proliferation between sheep that had been exposed to Mptb and became infected and those that were disease-free at the termination of the study. However, jejunal lymph node cells of Mptb-exposed infected animals had a significantly lower antigen-specific proliferative response compared to exposed uninfected animals. This may be useful when assessing JD status in animals of high commercial value. Multibacillary sheep had a lower proliferative response in peripheral blood compared to paucibacillary animals as early as 8 months p.i. and this was significantly different at 13 months p.i.