| Literature DB >> 19264122 |
Ming-Fen Song1, Yun-Shan Li, Yuko Ootsuyama, Hiroshi Kasai, Kazuaki Kawai, Masanori Ohta, Yasumasa Eguchi, Hiroshi Yamato, Yuki Matsumoto, Rie Yoshida, Yasutaka Ogawa.
Abstract
Urinary 8-OH-dG is commonly analyzed as a marker of oxidative stress. For its analysis, ELISA and HPLC methods are generally used, although discrepancies in the data obtained by these methods have often been discussed. To clarify this problem, we fractionated human urine by reverse-phase HPLC and assayed each fraction by the ELISA method. In addition to the 8-OH-dG fraction, a positive reaction was observed in the first eluted fraction. The components in this fraction were examined by the ELISA. Urea was found to be the responsible component in this fraction. Urea is present in high concentrations in the urine of mice, rats, and humans, and its level is influenced by many factors. Therefore, certain improvements, such as a correction based on urea content or urease treatment, are required for the accurate analysis of urinary 8-OH-dG by the ELISA method. In addition, performance of the ELISA at 4 degrees C reduced the recognition of urea considerably and improved the 8-OH-dG analysis.Entities:
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Year: 2009 PMID： 19264122 DOI： 10.1016/j.freeradbiomed.2009.02.017
Source DB: PubMed Journal: Free Radic Biol Med ISSN： 0891-5849 Impact factor: 7.376