OBJECTIVE AND DESIGN: To investigate the modulating role of lymphocytes in leukocyte recruitment in a murine model of peritonitis. MATERIALS OR SUBJECTS: RAG-1 knockout (KO) mice, NUDE mice and microMT KO mice were compared to their wild-type controls. TREATMENT: Mice were administered with 1 ml of Brewer's thioglycollate (BTG) and terminal peritoneal lavages were performed at 8, 24, 72 and 120 h after treatment. METHODS: Leukocyte numbers recruited at the different time points following a BTG administration were determined. Chemokine and cytokine levels were assessed by either ELISAs or cytometric bead array. RESULTS: RAG-1 KO mice (absent B and T cells) exhibited increased early neutrophil infiltration and blunted late monocyte/macrophage infiltration. NUDE mice (absent T cells) exhibited both increased neutrophil and monocyte/macrophage infiltration. In contrast, microMT KO mice (absent B cells) demonstrated reduced influx of both neutrophils and monocyte/macrophages. Chemokine analysis revealed various differences in important chemokines. CONCLUSIONS: These data suggest that T cells act to suppress leukocyte recruitment while B cells promote leukocyte recruitment.
OBJECTIVE AND DESIGN: To investigate the modulating role of lymphocytes in leukocyte recruitment in a murine model of peritonitis. MATERIALS OR SUBJECTS:RAG-1 knockout (KO) mice, NUDE mice and microMT KO mice were compared to their wild-type controls. TREATMENT: Mice were administered with 1 ml of Brewer's thioglycollate (BTG) and terminal peritoneal lavages were performed at 8, 24, 72 and 120 h after treatment. METHODS: Leukocyte numbers recruited at the different time points following a BTG administration were determined. Chemokine and cytokine levels were assessed by either ELISAs or cytometric bead array. RESULTS:RAG-1 KO mice (absent B and T cells) exhibited increased early neutrophil infiltration and blunted late monocyte/macrophage infiltration. NUDE mice (absent T cells) exhibited both increased neutrophil and monocyte/macrophage infiltration. In contrast, microMT KO mice (absent B cells) demonstrated reduced influx of both neutrophils and monocyte/macrophages. Chemokine analysis revealed various differences in important chemokines. CONCLUSIONS: These data suggest that T cells act to suppress leukocyte recruitment while B cells promote leukocyte recruitment.
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