Literature DB >> 19255486

Crystallization and preliminary X-ray analysis of human liver alpha-enolase.

Juan Wang1, Yan Feng Zhou, Lan Fen Li, Xiao Dong Su.   

Abstract

Enolase is a multifunctional enzyme that plays important roles in many biological and disease processes. alpha-Enolase from human liver (hENO1) was expressed as a soluble protein and purified by affinity column chromatography and gel filtration. Crystals were obtained by the hanging-drop vapour-diffusion method and diffracted to 2.5 A resolution. The crystals belonged to space group P2(1), with unit-cell parameters a = 72.85, b = 66.02, c = 79.43 A, beta = 94.54 degrees .

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Year:  2009        PMID： 19255486      PMCID： PMC2650463          DOI： 10.1107/S1744309109004138

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  15 in total

Review 1.  [SWISS-PROT: the curated protein sequence database on Internet].

Authors:  K Watanabe; S Harayama
Journal:  Tanpakushitsu Kakusan Koso       Date:  2001-01

2.  Plasmin(ogen)-binding alpha-enolase from Streptococcus pneumoniae: crystal structure and evaluation of plasmin(ogen)-binding sites.

Authors:  Stefanie Ehinger; Wolf-Dieter Schubert; Simone Bergmann; Sven Hammerschmidt; Dirk W Heinz
Journal:  J Mol Biol       Date:  2004-10-29       Impact factor: 5.469

3.  Crystal structure of enolase indicates that enolase and pyruvate kinase evolved from a common ancestor.

Authors:  L Lebioda; B Stec
Journal:  Nature       Date:  1988-06-16       Impact factor: 49.962

4.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

5.  Refined structure of yeast apo-enolase at 2.25 A resolution.

Authors:  B Stec; L Lebioda
Journal:  J Mol Biol       Date:  1990-01-05       Impact factor: 5.469

Review 6.  Structural and mechanistic studies of enolase.

Authors:  G H Reed; R R Poyner; T M Larsen; J E Wedekind; I Rayment
Journal:  Curr Opin Struct Biol       Date:  1996-12       Impact factor: 6.809

7.  Octahedral coordination at the high-affinity metal site in enolase: crystallographic analysis of the MgII--enzyme complex from yeast at 1.9 A resolution.

Authors:  J E Wedekind; G H Reed; I Rayment
Journal:  Biochemistry       Date:  1995-04-04       Impact factor: 3.162

8.  Structure of human alpha-enolase (hENO1), a multifunctional glycolytic enzyme.

Authors:  Hyo Jin Kang; Suk-Kyeong Jung; Seung Jun Kim; Sang J Chung
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2008-05-14

9.  Expression, purification and the 1.8 angstroms resolution crystal structure of human neuron specific enolase.

Authors:  Geqing Chai; John M Brewer; Leslie L Lovelace; Takashi Aoki; Wladek Minor; Lukasz Lebioda
Journal:  J Mol Biol       Date:  2004-08-20       Impact factor: 5.469

10.  A carboxylate oxygen of the substrate bridges the magnesium ions at the active site of enolase: structure of the yeast enzyme complexed with the equilibrium mixture of 2-phosphoglycerate and phosphoenolpyruvate at 1.8 A resolution.

Authors:  T M Larsen; J E Wedekind; I Rayment; G H Reed
Journal:  Biochemistry       Date:  1996-04-09       Impact factor: 3.162
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