Differential regulation of urocortins1-3 mRNA in human umbilical vein endothelial cells.

Urocortins (Ucns) are members of the corticotropin-releasing factor (CRF) family of peptides. Ucns would have potent effects on the cardiovascular system via the CRF receptor type 2 (CRF(2) receptor) in the cardiovascular system. However, an endogenous role and regulation of each Ucn have not been determined in the system. In the present study, we extended observations on stress or hormone-induced changes in Ucn gene expression in the cardiovascular system. Human umbilical vein endothelial cells (HUVECs) express Ucn1, Ucn2, and Ucn3 mRNAs, but not CRF mRNA, and the receptor, CRF(2 alpha) receptor mRNA. Lipopolysaccharides decreased Ucn1 mRNA levels, while it increased Ucn2 and Ucn3 mRNA levels in HUVECs. Interleukin-1beta decreased Ucn1 and Ucn2 mRNA levels, while it increased Ucn3 mRNA levels in HUVECs. Forskolin increased Ucn1 mRNA levels, while it decreased Ucn2 and Ucn3 mRNA levels. Ucns1-3 mRNA levels are differentially regulated in HUVECs. Differential regulation of Ucns1-3 mRNA may suggest differential roles of those in HUVECs.