Literature DB >> 1925306

Adherence of Helicobacter pylori to gastric carcinoma cells: analysis by flow cytometry.

B E Dunn1, M Altmann, G P Campbell.   

Abstract

An in vitro assay using immunofluorescence and flow cytometry for quantitative assessment of the adherence of Helicobacter pylori to cultured human gastric carcinoma (KATO III) cells was developed. Adherence was rapid, saturable, energy dependent, mannose resistant, and significantly inhibited by fetuin, a glycoprotein containing N-acetylneuraminyllactose. Pretreatment of KATO cells with neuraminidase from Clostridium perfringens, however, did not reduce adherence of H. pylori. Ultrastructurally, adherent cells of H. pylori were associated with indentations of KATO cell membranes. KATO cells should prove useful in the investigation of mechanisms of adherence of H. pylori to mammalian cells. Ultimately, this flow cytometric assay may be helpful in assessment of the adherence of laboratory strains of H. pylori directly to surface mucous cells dissociated from biopsied human gastric tissue.

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Mesh：

Year:  1991        PMID： 1925306     DOI： 10.1093/clinids/13.supplement_8.s657

Source DB:  PubMed          Journal:  Rev Infect Dis        ISSN： 0162-0886


  9 in total

1.  Flow cytometric analysis for adhesion of Vibrio cholerae to human intestinal epithelial cell.

Authors:  H Taguchi; H Yamaguchi; T Y Osaki; T Yamamoto; S Ogata; S Kamiya
Journal:  Eur J Epidemiol       Date:  1997-09       Impact factor: 8.082

2.  Effect of physiological concentrations of vitamin C on gastric cancer cells and Helicobacter pylori.

Authors:  Z-W Zhang; M Abdullahi; M J G Farthing
Journal:  Gut       Date:  2002-02       Impact factor: 23.059

3.  Adhesion of Helicobacter pylori to polarized T84 human intestinal cell monolayers is pH dependent.

Authors:  I Corthésy-Theulaz; N Porta; E Pringault; L Racine; A Bogdanova; J P Kraehenbuhl; A L Blum; P Michetti
Journal:  Infect Immun       Date:  1996-09       Impact factor: 3.441

4.  Inhibition of Helicobacter pylori binding to gastrointestinal epithelial cells by sialic acid-containing oligosaccharides.

Authors:  P M Simon; P L Goode; A Mobasseri; D Zopf
Journal:  Infect Immun       Date:  1997-02       Impact factor: 3.441

5.  Cell surface glycosaminoglycans are not involved in the adherence of Helicobacter pylori to cultured Hs 198.St human gastric cells, Hs 746T human gastric adenocarcinoma cells, or HeLa cells.

Authors:  H Kawasaki; G Sugumaran; J E Silbert
Journal:  Glycoconj J       Date:  1996-10       Impact factor: 2.916

6.  Helicobacter pylori pore-forming cytolysin orthologue TlyA possesses in vitro hemolytic activity and has a role in colonization of the gastric mucosa.

Authors:  M C Martino; R A Stabler; Z W Zhang; M J Farthing; B W Wren; N Dorrell
Journal:  Infect Immun       Date:  2001-03       Impact factor: 3.441

7.  Helicobacter pylori promotes the production of thymic stromal lymphopoietin by gastric epithelial cells and induces dendritic cell-mediated inflammatory Th2 responses.

Authors:  Masahiro Kido; Junya Tanaka; Nobuhiro Aoki; Satoru Iwamoto; Hisayo Nishiura; Tsutomu Chiba; Norihiko Watanabe
Journal:  Infect Immun       Date:  2009-10-19       Impact factor: 3.441

8.  Interleukin-8 response of gastric epithelial cell lines to Helicobacter pylori stimulation in vitro.

Authors:  S A Sharma; M K Tummuru; G G Miller; M J Blaser
Journal:  Infect Immun       Date:  1995-05       Impact factor: 3.441

9.  The effect of class II major histocompatibility complex expression on adherence of Helicobacter pylori and induction of apoptosis in gastric epithelial cells: a mechanism for T helper cell type 1-mediated damage.

Authors:  X Fan; S E Crowe; S Behar; H Gunasena; G Ye; H Haeberle; N Van Houten; W K Gourley; P B Ernst; V E Reyes
Journal:  J Exp Med       Date:  1998-05-18       Impact factor: 14.307
  9 in total

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