| Literature DB >> 19249788 |
Vanessa Gisele Pasqualotto Severino1, Cristiane de Melo Cazal, Moacir Rossi Forim, Maria Fátima das Graças Fernandes da Silva, Edson Rodrigues-Filho, João Batista Fernandes, Paulo Cezar Vieira.
Abstract
High-speed counter-current chromatography (HSCCC) with a two-phase solvent system (hexane-ethanol-acetonitrile-water 10:8:1:1, v/v) was applied to examine the leaves of Hortia oreadica, which afforded the known limonoid guyanin (1), the alkaloids rutaecarpin (2) and dictamnine (6), the dihydrocinnamic acid derivatives methyl 5,7-dimethoxy-2,2-dimethyl-2H-1-benzopyran-6-propanoate (3), 5,8-dimethoxy-2,2-dimethyl-2H-1-benzopyran-6-propanoic acid (4), together with the new E-3,4-dimethoxy-alpha(3-hydroxy-4-carbomethoxyphenyl)cinnamic acid (5). The recovery of compounds 1-6 was determined by comparison with LC-atmospheric pressure chemical ionization MS/MS data: 66.2%, 93.1%, 102.5%, 101.2%, 99.0% and 84.9%, respectively. Compound 3 showed IC(50) of 23.6microM against Plasmodium falciparum and 15.6microM against Trypanosoma brucei rhodesienses and was not toxic to KB cells (IC(50)>100microM).Entities:
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Year: 2009 PMID： 19249788 DOI： 10.1016/j.chroma.2009.02.009
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759