[Quantitative measurement of Fe(II) in human blood with spectral analysis method].

Since the absorption spectra of Fe(II) ions solutions vary with the concentration of Fe(II) irons, the authors tried to establish a direct measurement of active haemachrome in human blood through spectral analysis, and successfully made an experiment on it. The authors prepared Fe(II) ions solutions with various concentrations chemically, using 1, 10-phenanthroline (phen) as chromogenic agent. After the absorption spectra of these solutions were analyzed, Gaussion fitting at absorption peaks was performed to them. The linear fitting figure showed that the areas of Gaussion fitting curves correspond to the concentrations of Fe(II) irons. Thus, the concentration of Fe(II) in human blood was figured out in this way, and the result fitted quite well the actual concentration. As an optimized method, this creative idea is more accurate and precise. It will probably contribute to the development of new devices for measuring Fe(II) ions in human blood.