Literature DB >> 19247689

Saline extract of Taenia saginata metacestodes as an alternative antigen for the immunodiagnosis of neurocysticercosis in human cerebrospinal fluid.

Heliana B Oliveira1, Gleyce A Machado, Maria do Rosário F Gonçalves-Pires, Leandro P Moura, Julia M Costa-Cruz.   

Abstract

The aim of the present research was to test the application of Taenia saginata metacestodes as an alternative antigen for use in enzyme-linked immunosorbent assay (ELISA) and Western Blotting (WB) tests compared with the use of metacestodes antigen of Taenia solium in cerebrospinal fluid (CSF) samples. The samples were obtained from 35 patients with definitive neurocysticercosis (NCC)-group 1-and 44 patients with other neurological disorders (control)-group 2. The sensitivity and specificity of ELISA, using antigen obtained from T. solium, applied to the patients of group 1 yielded results of 100%. When the tests were conducted using T. saginata metacestodes, results were 100% and 93.2%, respectively. The 47-52-, 64-68-, and 70-kDa antigens showed high frequencies in CSF samples from patients with NCC when WB was conducted with both antigens. The results indicate that T. saginata metacestodes can be used as an alternative antigen for the diagnosis of human NCC in CSF samples.

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Year:  2009        PMID： 19247689     DOI： 10.1007/s00436-009-1379-z

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  31 in total

1.  Neurocysticercosis and population movements: analysis of 23 imported cases in Spain.

Authors:  C Roca; J Gascón; B Font; T Pujol; M E Valls; M Corachán
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2003-05-29       Impact factor: 3.267

2.  Taenia solium cysticercosis in Bali, Indonesia: serology and mtDNA analysis.

Authors:  A A R Sudewi; T Wandra; A Artha; A Nkouawa; A Ito
Journal:  Trans R Soc Trop Med Hyg       Date:  2007-10-24       Impact factor: 2.184

Review 3.  New concepts in the diagnosis and management of neurocysticercosis (Taenia solium).

Authors:  Hector H Garcia; Oscar H Del Brutto; Theodore E Nash; A Clinton White; Victor C W Tsang; Robert H Gilman
Journal:  Am J Trop Med Hyg       Date:  2005-01       Impact factor: 2.345

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 5.  Progress in diagnosis, treatment and elimination of echinococcosis and cysticercosis.

Authors:  Peter M Schantz
Journal:  Parasitol Int       Date:  2006-01-04       Impact factor: 2.230

6.  An epidemiological study of familial neurocysticercosis in an endemic Mexican community.

Authors:  A Fleury; J Morales; R J Bobes; M Dumas; O Yánez; J Piña; R Carrillo-Mezo; J J Martínez; G Fragoso; A Dessein; C Larralde; E Sciutto
Journal:  Trans R Soc Trop Med Hyg       Date:  2005-11-28       Impact factor: 2.184

7.  Use of Taenia crassiceps cysticercus antigen preparations for detection of antibodies in cerebrospinal fluid samples from patients with neurocysticercosis (Taenia solium).

Authors:  Alessandra Xavier Pardini; Regina Helena Peralta; Adelaide José Vaz; Luis dos Ramos Machado; José Mauro Peralta
Journal:  Clin Diagn Lab Immunol       Date:  2002-01

8.  Taenia solium metacestode immunodominant peptides recognized by IgG antibodies in cerebrospinal fluid and serum paired samples from patients with active and inactive neurocysticercosis.

Authors:  Ivanildes Solange da Costa Barcelos; Leandro Pajuaba de Moura; Vinicius Paulino da Costa; Marcelo Simão Ferreira; Julia Maria Costa-Cruz
Journal:  Mem Inst Oswaldo Cruz       Date:  2007-09       Impact factor: 2.743

Review 9.  Diagnostic criteria for neurocysticercosis: some modifications are needed for Indian patients.

Authors:  Ravindra Kumar Garg
Journal:  Neurol India       Date:  2004-06       Impact factor: 2.117

10.  Application of Taenia saginata metacestodes as an alternative antigen for the serological diagnosis of human neurocysticercosis.

Authors:  Heliana B Oliveira; Gleyce A Machado; Dagmar D Cabral; Julia M Costa-Cruz
Journal:  Parasitol Res       Date:  2007-05-19       Impact factor: 2.289
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  3 in total

1.  Taenia saginata metacestode antigenic fractions without affinity to concanavalin A are an important source of specific antigens for the diagnosis of human neurocysticercosis.

Authors:  Heliana B Oliveira; Gleyce A Machado; José R Mineo; Julia M Costa-Cruz
Journal:  Clin Vaccine Immunol       Date:  2010-02-03

Review 2.  Antiparasitic treatment of cerebral cysticercosis: lessons and experiences from China.

Authors:  Wei Wu; Fengju Jia; Wei Wang; Yixin Huang; Yong Huang
Journal:  Parasitol Res       Date:  2013-05-22       Impact factor: 2.289

Review 3.  Immunodiagnosis of neurocysticercosis: ways to focus on the challenge.

Authors:  M Esquivel-Velázquez; P Ostoa-Saloma; J Morales-Montor; R Hernández-Bello; C Larralde
Journal:  J Biomed Biotechnol       Date:  2011-10-29
  3 in total

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